Distinguishing Alternative Reaction Pathways by Single-Molecule Fluorescence Spectroscopy†
Arina Rybina
Cellnetworks Cluster & Physikalisch-Chemisches Institut, Universität Heidelberg, Im Neuenheimer Feld 267, 69120 Heidelberg (Germany)
Search for more papers by this authorDr. Carolin Lang
Organisch-Chemisches Institut, Universität Heidelberg, Im Neuenheimer Feld 270, 69120 Heidelberg (Germany)
Search for more papers by this authorMarcel Wirtz
Biophysikalische Chemie, Universität des Saarlandes, Campus Geb. B2.2, 66123 Saarbrücken (Germany)
Search for more papers by this authorKristin Grußmayer
Cellnetworks Cluster & Physikalisch-Chemisches Institut, Universität Heidelberg, Im Neuenheimer Feld 267, 69120 Heidelberg (Germany)
Search for more papers by this authorDr. Anton Kurz
Cellnetworks Cluster & Physikalisch-Chemisches Institut, Universität Heidelberg, Im Neuenheimer Feld 267, 69120 Heidelberg (Germany)
Search for more papers by this authorFrank Maier
Organisch-Chemisches Institut, Universität Heidelberg, Im Neuenheimer Feld 270, 69120 Heidelberg (Germany)
Search for more papers by this authorDr. Alexander Schmitt
Biophysikalische Chemie, Universität des Saarlandes, Campus Geb. B2.2, 66123 Saarbrücken (Germany)
Search for more papers by this authorProf. Oliver Trapp
Organisch-Chemisches Institut, Universität Heidelberg, Im Neuenheimer Feld 270, 69120 Heidelberg (Germany)
Search for more papers by this authorProf. Gregor Jung
Biophysikalische Chemie, Universität des Saarlandes, Campus Geb. B2.2, 66123 Saarbrücken (Germany)
Search for more papers by this authorCorresponding Author
Dr. Dirk-Peter Herten
Cellnetworks Cluster & Physikalisch-Chemisches Institut, Universität Heidelberg, Im Neuenheimer Feld 267, 69120 Heidelberg (Germany)
Cellnetworks Cluster & Physikalisch-Chemisches Institut, Universität Heidelberg, Im Neuenheimer Feld 267, 69120 Heidelberg (Germany)Search for more papers by this authorArina Rybina
Cellnetworks Cluster & Physikalisch-Chemisches Institut, Universität Heidelberg, Im Neuenheimer Feld 267, 69120 Heidelberg (Germany)
Search for more papers by this authorDr. Carolin Lang
Organisch-Chemisches Institut, Universität Heidelberg, Im Neuenheimer Feld 270, 69120 Heidelberg (Germany)
Search for more papers by this authorMarcel Wirtz
Biophysikalische Chemie, Universität des Saarlandes, Campus Geb. B2.2, 66123 Saarbrücken (Germany)
Search for more papers by this authorKristin Grußmayer
Cellnetworks Cluster & Physikalisch-Chemisches Institut, Universität Heidelberg, Im Neuenheimer Feld 267, 69120 Heidelberg (Germany)
Search for more papers by this authorDr. Anton Kurz
Cellnetworks Cluster & Physikalisch-Chemisches Institut, Universität Heidelberg, Im Neuenheimer Feld 267, 69120 Heidelberg (Germany)
Search for more papers by this authorFrank Maier
Organisch-Chemisches Institut, Universität Heidelberg, Im Neuenheimer Feld 270, 69120 Heidelberg (Germany)
Search for more papers by this authorDr. Alexander Schmitt
Biophysikalische Chemie, Universität des Saarlandes, Campus Geb. B2.2, 66123 Saarbrücken (Germany)
Search for more papers by this authorProf. Oliver Trapp
Organisch-Chemisches Institut, Universität Heidelberg, Im Neuenheimer Feld 270, 69120 Heidelberg (Germany)
Search for more papers by this authorProf. Gregor Jung
Biophysikalische Chemie, Universität des Saarlandes, Campus Geb. B2.2, 66123 Saarbrücken (Germany)
Search for more papers by this authorCorresponding Author
Dr. Dirk-Peter Herten
Cellnetworks Cluster & Physikalisch-Chemisches Institut, Universität Heidelberg, Im Neuenheimer Feld 267, 69120 Heidelberg (Germany)
Cellnetworks Cluster & Physikalisch-Chemisches Institut, Universität Heidelberg, Im Neuenheimer Feld 267, 69120 Heidelberg (Germany)Search for more papers by this authorWe thank the Deutsche Forschungsgemeinschaft (DFG) for their financial support (EXC81, SFB623). We also acknowledge Stephen Hashmi (Heidelberg University) for fruitful discussions. Volker Huch is gratefully acknowledged for X-ray crystallography. Michael Schwering and Dominik Brox have continuously supported the project with their expertise in microscopy.
Graphical Abstract
Focus on chemical transitions: Epoxidation of a double bond in conjugation to a fluorescent dye was studied at single-molecule level. Direct observation of oxirane formation, indicated as a spectral shift from substrate to product state, revealed an alternative reaction pathway for the epoxidation reaction.
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