Simultaneous Measurement of SUMOylation using SNAP/CLIP-Tag-Mediated Translation at the Single-Molecule Level†
Dr. Yong Yang
Single-molecule Detection and Imaging Laboratory, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Guangdong, 518055 (China)
Search for more papers by this authorCorresponding Author
Prof. Dr. Chun-yang Zhang
Single-molecule Detection and Imaging Laboratory, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Guangdong, 518055 (China)
Single-molecule Detection and Imaging Laboratory, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Guangdong, 518055 (China)Search for more papers by this authorDr. Yong Yang
Single-molecule Detection and Imaging Laboratory, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Guangdong, 518055 (China)
Search for more papers by this authorCorresponding Author
Prof. Dr. Chun-yang Zhang
Single-molecule Detection and Imaging Laboratory, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Guangdong, 518055 (China)
Single-molecule Detection and Imaging Laboratory, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Guangdong, 518055 (China)Search for more papers by this authorThis work was supported by the National Basic Research Program 973 (Grant Nos. 2011CB933600 and 2010CB732600), the Award for the Hundred Talent Program of the Chinese Academy of Science, the Natural Science Foundation of China (Grant Nos. 21075129 and 31000599), the Guangdong Innovation Research Team Fund for Low-cost Healthcare Technologies, the Natural Science Foundation of Shenzhen City (Grant Nos. JC201005270327A and JC201005270355A), and the Fund for Shenzhen Engineering Laboratory of Single-molecule Detection and Instrument Development (Grant No. (2012) 433). SUMO=small ubiquitin-like modifier.
Graphical Abstract
Two opposing SUMO wrestlers: Simultaneous measurement of multiple SUMOylations was achieved at the single-molecule level by integrating SNAP/CLIP-tag-mediated translation with single-molecule detection (see scheme; SUMO=small ubiquitin-like modifier). This method gives exceptional sensitivity, and is capable of measuring the SUMOylation of different proteins under various physiological conditions.
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