DNA-Coated AFM Cantilevers for the Investigation of Cell Adhesion and the Patterning of Live Cells†
Sonny C. Hsiao
Department of Chemistry, University of California, Berkeley
Materials Sciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720 (USA), Fax: (+1) 510-643-3079
Search for more papers by this authorAiley K. Crow
Biophysics Graduate Group, University of California, Berkeley (USA)
Search for more papers by this authorWilbur A. Lam
UCB/UCSF Joint Graduate Group in Bioengineering, University of California, Berkeley (USA)
Search for more papers by this authorCarolyn R. Bertozzi Prof.
Department of Chemistry & Molecular and Cell Biology
Howard Hughes Medical Institute, University of California, Berkeley
Materials Sciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720 (USA)
Search for more papers by this authorDaniel A. Fletcher Prof.
Biophysics Graduate Group, University of California, Berkeley
UCB/UCSF Joint Graduate Group in Bioengineering, University of California, Berkeley (USA)
Search for more papers by this authorMatthew B. Francis Prof.
Department of Chemistry, University of California, Berkeley
Materials Sciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720 (USA), Fax: (+1) 510-643-3079
Search for more papers by this authorSonny C. Hsiao
Department of Chemistry, University of California, Berkeley
Materials Sciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720 (USA), Fax: (+1) 510-643-3079
Search for more papers by this authorAiley K. Crow
Biophysics Graduate Group, University of California, Berkeley (USA)
Search for more papers by this authorWilbur A. Lam
UCB/UCSF Joint Graduate Group in Bioengineering, University of California, Berkeley (USA)
Search for more papers by this authorCarolyn R. Bertozzi Prof.
Department of Chemistry & Molecular and Cell Biology
Howard Hughes Medical Institute, University of California, Berkeley
Materials Sciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720 (USA)
Search for more papers by this authorDaniel A. Fletcher Prof.
Biophysics Graduate Group, University of California, Berkeley
UCB/UCSF Joint Graduate Group in Bioengineering, University of California, Berkeley (USA)
Search for more papers by this authorMatthew B. Francis Prof.
Department of Chemistry, University of California, Berkeley
Materials Sciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720 (USA), Fax: (+1) 510-643-3079
Search for more papers by this authorWe thank Z. Gartner, R. Chandra, E. Douglas, and X. Chen for technical assistance and helpful discussions, and especially Z. Gartner for DNA preparation. This work was generously supported by Chemical Sciences, Geosciences, and Biosciences Division, Office of Basic Energy Sciences of the US Department of Energy under Contract No. DE-AC03-76SF00098, a National Research Service Award of National Institutes of Health, and a Hammond Research Fellowship of the National Childhood Cancer Foundation and Children's Oncology Group to W.A.L.
Graphical Abstract
Get a grip: Measurement of receptor adhesion strength requires the precise manipulation of single cells on a contact surface. To attach live cells to a moveable probe, DNA sequences complementary to strands displayed on the plasma membrane are introduced onto AFM cantilevers (see picture, bp=base pairs). The strength of the resulting linkages can be tuned by varying the length of DNA strands, allowing for controlled transport of the cells.
Supporting Information
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| anie_200802525_sm_miscellaneous_information.pdf557.2 KB | miscellaneous information |
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