Communication

Homogeneous Biocatalysis in both Fluorous Biphasic and Supercritical Carbon Dioxide Systems^†

Helen R. Hobbs Dr.

School of Chemistry, The University of Nottingham, University Park, Nottingham, NG7 2RD, UK, Fax: (+44) 115-951-3564 http://www.nottingham.ac.uk/supercritical

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Helen M. Kirke Dr.,

Helen M. Kirke Dr.

School of Chemistry, The University of Nottingham, University Park, Nottingham, NG7 2RD, UK, Fax: (+44) 115-951-3564 http://www.nottingham.ac.uk/supercritical

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Martyn Poliakoff Prof.,

Martyn Poliakoff Prof.

School of Chemistry, The University of Nottingham, University Park, Nottingham, NG7 2RD, UK, Fax: (+44) 115-951-3564 http://www.nottingham.ac.uk/supercritical

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Neil R. Thomas Dr.,

Neil R. Thomas Dr.

[email protected]

School of Chemistry, The University of Nottingham, University Park, Nottingham, NG7 2RD, UK, Fax: (+44) 115-951-3564 http://www.nottingham.ac.uk/supercritical

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Helen R. Hobbs Dr.,

Helen R. Hobbs Dr.

School of Chemistry, The University of Nottingham, University Park, Nottingham, NG7 2RD, UK, Fax: (+44) 115-951-3564 http://www.nottingham.ac.uk/supercritical

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Helen M. Kirke Dr.,

Helen M. Kirke Dr.

School of Chemistry, The University of Nottingham, University Park, Nottingham, NG7 2RD, UK, Fax: (+44) 115-951-3564 http://www.nottingham.ac.uk/supercritical

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Martyn Poliakoff Prof.,

Martyn Poliakoff Prof.

School of Chemistry, The University of Nottingham, University Park, Nottingham, NG7 2RD, UK, Fax: (+44) 115-951-3564 http://www.nottingham.ac.uk/supercritical

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Neil R. Thomas Dr.,

Neil R. Thomas Dr.

[email protected]

School of Chemistry, The University of Nottingham, University Park, Nottingham, NG7 2RD, UK, Fax: (+44) 115-951-3564 http://www.nottingham.ac.uk/supercritical

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First published: 04 October 2007

https://doi.org/10.1002/anie.200701488

Citations: 24

^†

We thank Dr. Ludo Kleintjens (DSM Research, Geleen) and Prof. Eric Beckman (University of Pittsburgh) for helpful scientific discussions, Dr. Michael Kaszuba (Malvern Instruments) for assistance with the DLS measurements, Dr. Paul Blood (SChEME, University of Nottingham) for assistance with the online video, and DSM, BBSRC, the EPSRC, and the Royal Society for financial support.

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Graphical Abstract

HIP to be green: Cytochrome c and α-chymotrypsin (CMT) can be solubilized in either fluorous solvents or supercritical CO₂ by hydrophobic ion pairing (HIP) with perfluorinated anionic surfactants (Krytox 157 FSL, KDP 4606). A model system for homogeneous fluorous biphasic biocatalysis involving the CMT–KDP complex in hexane/perfluoromethylcyclohexane is reported that simplifies both product separation and recycling of the biocatalyst.

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Volume46, Issue41

October 15, 2007

Pages 7860-7863

Homogeneous Biocatalysis in both Fluorous Biphasic and Supercritical Carbon Dioxide Systems^†

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Homogeneous Biocatalysis in both Fluorous Biphasic and Supercritical Carbon Dioxide Systems†

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Homogeneous Biocatalysis in both Fluorous Biphasic and Supercritical Carbon Dioxide Systems^†