Volume 137, Issue 12 e202581261
Frontispiz
Free Access

Frontispiz: Ultrasensitive Characterization of Native Bacterial Biofilms via Dynamic Nuclear Polarization-Enhanced Solid-State NMR

Chang-Hyeock Byeon

Chang-Hyeock Byeon

Department of Structural Biology, School of Medicine, University of Pittsburgh, Pittsburgh, PA, 15261 USA

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Ted Kinney

Ted Kinney

Department of Structural Biology, School of Medicine, University of Pittsburgh, Pittsburgh, PA, 15261 USA

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Dr. Hakan Saricayir

Dr. Hakan Saricayir

Department of Structural Biology, School of Medicine, University of Pittsburgh, Pittsburgh, PA, 15261 USA

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Kasper Holst Hansen

Kasper Holst Hansen

Department of Structural Biology, School of Medicine, University of Pittsburgh, Pittsburgh, PA, 15261 USA

Department of Biomedicine, Aarhus University, Aarhus, 8000 Denmark

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Dr. Faith J. Scott

Dr. Faith J. Scott

National High Magnetic Field Laboratory, Florida State University, Tallahassee, FL, 32310 USA

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Sadhana Srinivasa

Sadhana Srinivasa

Department of Biological Sciences, Duquesne University, Pittsburgh, PA, 15282 USA

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Meghan K. Wells

Meghan K. Wells

Department of Biological Sciences, Duquesne University, Pittsburgh, PA, 15282 USA

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Dr. Frederic Mentink-Vigier

Dr. Frederic Mentink-Vigier

National High Magnetic Field Laboratory, Florida State University, Tallahassee, FL, 32310 USA

Department of Chemistry and Biochemistry, Florida State University, Tallahassee, FL, 32306 USA

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Prof. Wook Kim

Prof. Wook Kim

Department of Biological Sciences, Duquesne University, Pittsburgh, PA, 15282 USA

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Prof. Ümit Akbey

Corresponding Author

Prof. Ümit Akbey

Department of Structural Biology, School of Medicine, University of Pittsburgh, Pittsburgh, PA, 15261 USA

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First published: 18 March 2025

Graphical Abstract

Ultrasensitive characterization of native bacterial biofilm: A high-sensitivity dynamic nuclear polarization (DNP) enhanced solid-state NMR method illuminates the composition of intact native bacterial biofilm. The DNP sensitivity boost (~75-fold) allows observing NMR signals of biofilms composed of bacteria and extracellular matrix at natural abundance, shown here for Pseudomonas. Chemical identification quantifies the biofilm components, as reported by Ümit Akbey et^^al. in their Research Article (e202418146).

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