Fast Antioxidation Kinetics of Glutathione Intracellularly Monitored by a Dual-Wire Nanosensor
Yu-Ting Jiao
College of Chemistry and Molecular Sciences, Wuhan University, Wuhan, 430072 China
Search for more papers by this authorYi-Ran Kang
College of Chemistry and Molecular Sciences, Wuhan University, Wuhan, 430072 China
Search for more papers by this authorMing-Yong Wen
College of Chemistry and Molecular Sciences, Wuhan University, Wuhan, 430072 China
Search for more papers by this authorHui-Qian Wu
College of Chemistry and Molecular Sciences, Wuhan University, Wuhan, 430072 China
Search for more papers by this authorCorresponding Author
Prof. Dr. Xin-Wei Zhang
College of Chemistry and Molecular Sciences, Wuhan University, Wuhan, 430072 China
Search for more papers by this authorCorresponding Author
Prof. Dr. Wei-Hua Huang
College of Chemistry and Molecular Sciences, Wuhan University, Wuhan, 430072 China
Search for more papers by this authorYu-Ting Jiao
College of Chemistry and Molecular Sciences, Wuhan University, Wuhan, 430072 China
Search for more papers by this authorYi-Ran Kang
College of Chemistry and Molecular Sciences, Wuhan University, Wuhan, 430072 China
Search for more papers by this authorMing-Yong Wen
College of Chemistry and Molecular Sciences, Wuhan University, Wuhan, 430072 China
Search for more papers by this authorHui-Qian Wu
College of Chemistry and Molecular Sciences, Wuhan University, Wuhan, 430072 China
Search for more papers by this authorCorresponding Author
Prof. Dr. Xin-Wei Zhang
College of Chemistry and Molecular Sciences, Wuhan University, Wuhan, 430072 China
Search for more papers by this authorCorresponding Author
Prof. Dr. Wei-Hua Huang
College of Chemistry and Molecular Sciences, Wuhan University, Wuhan, 430072 China
Search for more papers by this authorAbstract
The glutathione (GSH) system is one of the most powerful intracellular antioxidant systems for the elimination of reactive oxygen species (ROS) and maintaining cellular redox homeostasis. However, the rapid kinetics information (at the millisecond to the second level) during the dynamic antioxidation process of the GSH system remains unclear. As such, we specifically developed a novel dual-wire nanosensor (DWNS) that can selectively and synchronously measure the levels of GSH and ROS with high temporal resolution, and applied it to monitor the transient ROS generation as well as the rapid antioxidation process of the GSH system in individual cancer cells. These measurements revealed that the glutathione peroxidase (GPx) in the GSH system is rapidly initiated against ROS burst in a sub-second time scale, but the elimination process is short-lived, ending after a few seconds, while some ROS are still present in the cells. This study is expected to open new perspectives for understanding the GSH antioxidant system and studying some redox imbalance-related physiological.
Open Research
Data Availability Statement
The data that support the findings of this study are available from the corresponding author upon reasonable request.
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