Volume 134, Issue 8 e202200436
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Rücktitelbild: Gene Fusion and Directed Evolution to Break Structural Symmetry and Boost Catalysis by an Oligomeric C−C Bond-Forming Enzyme (Angew. Chem. 8/2022)

Guangcai Xu

Guangcai Xu

Department of Chemical and Pharmaceutical Biology, Groningen Research Institute of Pharmacy, University of Groningen, Antonius Deusinglaan 1, 9713 AV Groningen, The Netherlands

These authors contributed equally to this work.

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Andreas Kunzendorf

Andreas Kunzendorf

Department of Chemical and Pharmaceutical Biology, Groningen Research Institute of Pharmacy, University of Groningen, Antonius Deusinglaan 1, 9713 AV Groningen, The Netherlands

These authors contributed equally to this work.

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Michele Crotti

Michele Crotti

Department of Chemical and Pharmaceutical Biology, Groningen Research Institute of Pharmacy, University of Groningen, Antonius Deusinglaan 1, 9713 AV Groningen, The Netherlands

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Henriëtte J. Rozeboom

Henriëtte J. Rozeboom

Molecular Enzymology Group, Groningen Institute of Biomolecular Sciences and Biotechnology, University of Groningen, Nijenborgh 4, 9747 AG Groningen, The Netherlands

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Andy-Mark W. H. Thunnissen

Andy-Mark W. H. Thunnissen

Molecular Enzymology Group, Groningen Institute of Biomolecular Sciences and Biotechnology, University of Groningen, Nijenborgh 4, 9747 AG Groningen, The Netherlands

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Gerrit J. Poelarends

Corresponding Author

Gerrit J. Poelarends

Department of Chemical and Pharmaceutical Biology, Groningen Research Institute of Pharmacy, University of Groningen, Antonius Deusinglaan 1, 9713 AV Groningen, The Netherlands

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First published: 18 January 2022

Graphical Abstract

Die Genfusion ist ein wichtiger natürlicher Prozess zur Erzeugung neuer Enzyme aus einfachen Vorläufern. Gerrit J. Poelarends und Mitarbeiter haben diese Strategie in ihrer Zuschrift (e202113970) angewendet, um eine promiskuitive homohexamere 4-Oxalocrotonat-Tautomerase (4-OT) zu einem effizienten Enzym für enantioselektive Michael-Reaktionen zu entwickeln. Sie entwarfen eine tandemfusionierte 4-OT mit reduzierter Symmetrie, die eine unabhängige Sequenzdiversifizierung benachbarter Untereinheiten ermöglicht und so den Proteinsequenzraum vergrößert, der durch gerichtete Evolution erforscht werden kann.

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