Visualizing Microglia with a Fluorescence Turn-On Ugt1a7c Substrate
Dr. Beomsue Kim
Singapore Bioimaging Consortium, Agency for Science Technology and Research (A*STAR), Singapore, 138667 Singapore
These authors contributed equally to this work.
Search for more papers by this authorDr. Masahiro Fukuda
Program in Neuroscience and Behavioral Disorders, Duke-NUS Medical School, Singapore, 169857 Singapore
These authors contributed equally to this work.
Search for more papers by this authorDr. Jung-Yeol Lee
Department of Chemistry, Pohang University of Science and Technology, Pohang, 37673 Korea
Singapore Bioimaging Consortium, Agency for Science Technology and Research (A*STAR), Singapore, 138667 Singapore
Present address: New drug discovery center, DGMIF, Daegu, 41061 Korea
Search for more papers by this authorDr. Dongdong Su
Singapore Bioimaging Consortium, Agency for Science Technology and Research (A*STAR), Singapore, 138667 Singapore
Search for more papers by this authorDr. Srikanta Sanu
Singapore Bioimaging Consortium, Agency for Science Technology and Research (A*STAR), Singapore, 138667 Singapore
Search for more papers by this authorDr. Aymeric Silvin
Singapore Immunology Network, A*STAR, Singapore, 138648 Singapore
Search for more papers by this authorAudrey T. T. Khoo
Program in Neuroscience and Behavioral Disorders, Duke-NUS Medical School, Singapore, 169857 Singapore
Search for more papers by this authorProf. Taejoon Kwon
Department of Biomedical Engineering, Ulsan National Institute of Science and Technology, Ulsan, 44919 Korea
Search for more papers by this authorXiao Liu
Department of Chemistry, Pohang University of Science and Technology, Pohang, 37673 Korea
Center for Self-assembly and Complexity, Institute for Basic Science (IBS), Pohang, 37673 Korea
Search for more papers by this authorDr. Weijie Chi
Singapore University of Technology and Design, Singapore, 487372 Singapore
Search for more papers by this authorProf. Xiaogang Liu
Singapore University of Technology and Design, Singapore, 487372 Singapore
Search for more papers by this authorSejong Choi
Department of Chemistry, Seoul National University, Seoul, 08826 Korea
Search for more papers by this authorDiana S. Y. Wan
Singapore Bioimaging Consortium, Agency for Science Technology and Research (A*STAR), Singapore, 138667 Singapore
Search for more papers by this authorDr. Sung-Jin Park
Singapore Bioimaging Consortium, Agency for Science Technology and Research (A*STAR), Singapore, 138667 Singapore
Search for more papers by this authorProf. Jin-Soo Kim
Center for Genome Engineering, IBS, Daejeon, 34047 Korea
Search for more papers by this authorProf. Florent Ginhoux
Singapore Immunology Network, A*STAR, Singapore, 138648 Singapore
Search for more papers by this authorCorresponding Author
Prof. H. Shawn Je
Program in Neuroscience and Behavioral Disorders, Duke-NUS Medical School, Singapore, 169857 Singapore
Search for more papers by this authorCorresponding Author
Prof. Young-Tae Chang
Department of Chemistry, Pohang University of Science and Technology, Pohang, 37673 Korea
Singapore Bioimaging Consortium, Agency for Science Technology and Research (A*STAR), Singapore, 138667 Singapore
Center for Self-assembly and Complexity, Institute for Basic Science (IBS), Pohang, 37673 Korea
Search for more papers by this authorDr. Beomsue Kim
Singapore Bioimaging Consortium, Agency for Science Technology and Research (A*STAR), Singapore, 138667 Singapore
These authors contributed equally to this work.
Search for more papers by this authorDr. Masahiro Fukuda
Program in Neuroscience and Behavioral Disorders, Duke-NUS Medical School, Singapore, 169857 Singapore
These authors contributed equally to this work.
Search for more papers by this authorDr. Jung-Yeol Lee
Department of Chemistry, Pohang University of Science and Technology, Pohang, 37673 Korea
Singapore Bioimaging Consortium, Agency for Science Technology and Research (A*STAR), Singapore, 138667 Singapore
Present address: New drug discovery center, DGMIF, Daegu, 41061 Korea
Search for more papers by this authorDr. Dongdong Su
Singapore Bioimaging Consortium, Agency for Science Technology and Research (A*STAR), Singapore, 138667 Singapore
Search for more papers by this authorDr. Srikanta Sanu
Singapore Bioimaging Consortium, Agency for Science Technology and Research (A*STAR), Singapore, 138667 Singapore
Search for more papers by this authorDr. Aymeric Silvin
Singapore Immunology Network, A*STAR, Singapore, 138648 Singapore
Search for more papers by this authorAudrey T. T. Khoo
Program in Neuroscience and Behavioral Disorders, Duke-NUS Medical School, Singapore, 169857 Singapore
Search for more papers by this authorProf. Taejoon Kwon
Department of Biomedical Engineering, Ulsan National Institute of Science and Technology, Ulsan, 44919 Korea
Search for more papers by this authorXiao Liu
Department of Chemistry, Pohang University of Science and Technology, Pohang, 37673 Korea
Center for Self-assembly and Complexity, Institute for Basic Science (IBS), Pohang, 37673 Korea
Search for more papers by this authorDr. Weijie Chi
Singapore University of Technology and Design, Singapore, 487372 Singapore
Search for more papers by this authorProf. Xiaogang Liu
Singapore University of Technology and Design, Singapore, 487372 Singapore
Search for more papers by this authorSejong Choi
Department of Chemistry, Seoul National University, Seoul, 08826 Korea
Search for more papers by this authorDiana S. Y. Wan
Singapore Bioimaging Consortium, Agency for Science Technology and Research (A*STAR), Singapore, 138667 Singapore
Search for more papers by this authorDr. Sung-Jin Park
Singapore Bioimaging Consortium, Agency for Science Technology and Research (A*STAR), Singapore, 138667 Singapore
Search for more papers by this authorProf. Jin-Soo Kim
Center for Genome Engineering, IBS, Daejeon, 34047 Korea
Search for more papers by this authorProf. Florent Ginhoux
Singapore Immunology Network, A*STAR, Singapore, 138648 Singapore
Search for more papers by this authorCorresponding Author
Prof. H. Shawn Je
Program in Neuroscience and Behavioral Disorders, Duke-NUS Medical School, Singapore, 169857 Singapore
Search for more papers by this authorCorresponding Author
Prof. Young-Tae Chang
Department of Chemistry, Pohang University of Science and Technology, Pohang, 37673 Korea
Singapore Bioimaging Consortium, Agency for Science Technology and Research (A*STAR), Singapore, 138667 Singapore
Center for Self-assembly and Complexity, Institute for Basic Science (IBS), Pohang, 37673 Korea
Search for more papers by this authorAbstract
Microglia, the brain-resident macrophage, are involved in brain development and contribute to the progression of neural disorders. Despite the importance of microglia, imaging of live microglia at a cellular resolution has been limited to transgenic mice. Efforts have therefore been dedicated to developing new methods for microglia detection and imaging. Using a thorough structure–activity relationships study, we developed CDr20, a high-performance fluorogenic chemical probe that enables the visualization of microglia both in vitro and in vivo. Using a genome-scale CRISPR-Cas9 knockout screen, the UDP-glucuronosyltransferase Ugt1a7c was identified as the target of CDr20. The glucuronidation of CDr20 by Ugt1a7c in microglia produces fluorescence.
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