A Fluorescent Reporter of the Phosphorylation Status of the Substrate Protein STAT3†
Vanessa K. Lacey
Jack H. Skirball Center for Chemical Biology & Proteomics, The Salk Institute for Biological Studies, 10010 N. Torrey Pines Road, La Jolla, CA 92037 (USA) http://wang.salk.edu/
Search for more papers by this authorAngela R. Parrish
Jack H. Skirball Center for Chemical Biology & Proteomics, The Salk Institute for Biological Studies, 10010 N. Torrey Pines Road, La Jolla, CA 92037 (USA) http://wang.salk.edu/
Search for more papers by this authorShuliang Han
College of Chemistry, Peking University, Beijing 100871 (China)
Search for more papers by this authorDr. Zhouxin Shen
Section of Cell and Development Biology, University of California at San Diego, La Jolla, CA 92037 (USA)
Search for more papers by this authorProf. Steven P. Briggs
Section of Cell and Development Biology, University of California at San Diego, La Jolla, CA 92037 (USA)
Search for more papers by this authorProf. Yuguo Ma
College of Chemistry, Peking University, Beijing 100871 (China)
Search for more papers by this authorCorresponding Author
Prof. Lei Wang
Jack H. Skirball Center for Chemical Biology & Proteomics, The Salk Institute for Biological Studies, 10010 N. Torrey Pines Road, La Jolla, CA 92037 (USA) http://wang.salk.edu/
Jack H. Skirball Center for Chemical Biology & Proteomics, The Salk Institute for Biological Studies, 10010 N. Torrey Pines Road, La Jolla, CA 92037 (USA) http://wang.salk.edu/Search for more papers by this authorVanessa K. Lacey
Jack H. Skirball Center for Chemical Biology & Proteomics, The Salk Institute for Biological Studies, 10010 N. Torrey Pines Road, La Jolla, CA 92037 (USA) http://wang.salk.edu/
Search for more papers by this authorAngela R. Parrish
Jack H. Skirball Center for Chemical Biology & Proteomics, The Salk Institute for Biological Studies, 10010 N. Torrey Pines Road, La Jolla, CA 92037 (USA) http://wang.salk.edu/
Search for more papers by this authorShuliang Han
College of Chemistry, Peking University, Beijing 100871 (China)
Search for more papers by this authorDr. Zhouxin Shen
Section of Cell and Development Biology, University of California at San Diego, La Jolla, CA 92037 (USA)
Search for more papers by this authorProf. Steven P. Briggs
Section of Cell and Development Biology, University of California at San Diego, La Jolla, CA 92037 (USA)
Search for more papers by this authorProf. Yuguo Ma
College of Chemistry, Peking University, Beijing 100871 (China)
Search for more papers by this authorCorresponding Author
Prof. Lei Wang
Jack H. Skirball Center for Chemical Biology & Proteomics, The Salk Institute for Biological Studies, 10010 N. Torrey Pines Road, La Jolla, CA 92037 (USA) http://wang.salk.edu/
Jack H. Skirball Center for Chemical Biology & Proteomics, The Salk Institute for Biological Studies, 10010 N. Torrey Pines Road, La Jolla, CA 92037 (USA) http://wang.salk.edu/Search for more papers by this authorWe thank Dr. Tony Hunter for helpful discussions. L.W. thanks the support from the Salk Innovation grant, March of Dimes Foundation (5-FY08-110), CIRM (RN1-00577-1), NCI (P30CA014195) and NIH (1DP2OD004744).
Graphical Abstract
Ein reversibler Biosensor gibt Auskunft über den Phosphorylierungszustand des Volllängen-Substratproteins STAT3, indem er genetisch 7-Hydroxycumarin in dessen Phosphotyrosin-Bindetasche einbaut (siehe Bild). Eine starke Zunahme der Fluoreszenz mit charakteristischen Emissions- und Anregungseigenschaften tritt bei Phosphorylierung von STAT3 in vitro und endogen in Zellkernextrakten auf.
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