Volume 123, Issue 37 pp. 8877-8880

Zuschrift

The 3D Solution Structure of Thurincin H, a Bacteriocin with Four Sulfur to α-Carbon Crosslinks^†

Clarissa S. Sit,

Clarissa S. Sit

Department of Chemistry, University of Alberta, Edmonton, Alberta, T6G 2G2 (Canada)

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Marco J. van Belkum,

Marco J. van Belkum

Department of Chemistry, University of Alberta, Edmonton, Alberta, T6G 2G2 (Canada)

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Ryan T. McKay,

Ryan T. McKay

National High Field NMR Centre (NANUC), University of Alberta, Edmonton, Alberta, T6G 2E1 (Canada)

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Prof. Randy W. Worobo,

Prof. Randy W. Worobo

Department of Food Science, New York State Agricultural Experiment Station, Cornell University, 630 W North St., Geneva, NY 14456 (USA)

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Prof. John C. Vederas,

Corresponding Author

Prof. John C. Vederas

[email protected]

Department of Chemistry, University of Alberta, Edmonton, Alberta, T6G 2G2 (Canada)

Department of Chemistry, University of Alberta, Edmonton, Alberta, T6G 2G2 (Canada)Search for more papers by this author

Clarissa S. Sit,

Clarissa S. Sit

Department of Chemistry, University of Alberta, Edmonton, Alberta, T6G 2G2 (Canada)

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Marco J. van Belkum,

Marco J. van Belkum

Department of Chemistry, University of Alberta, Edmonton, Alberta, T6G 2G2 (Canada)

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Ryan T. McKay,

Ryan T. McKay

National High Field NMR Centre (NANUC), University of Alberta, Edmonton, Alberta, T6G 2E1 (Canada)

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Prof. Randy W. Worobo,

Prof. Randy W. Worobo

Department of Food Science, New York State Agricultural Experiment Station, Cornell University, 630 W North St., Geneva, NY 14456 (USA)

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Prof. John C. Vederas,

Corresponding Author

Prof. John C. Vederas

[email protected]

Department of Chemistry, University of Alberta, Edmonton, Alberta, T6G 2G2 (Canada)

Department of Chemistry, University of Alberta, Edmonton, Alberta, T6G 2G2 (Canada)Search for more papers by this author

First published: 22 July 2011

https://doi.org/10.1002/ange.201102527

Citations: 9

^†

We thank Dr. Randy Whittal, Jing Zheng, and Bela Reiz for performing the mass spectrometry analysis. We thank Mark Miskolzie for advice regarding NMR experiments and data analysis. We thank Dr. Leah Martin-Visscher and Dr. Pascal Mercier for their assistance with CYANA. This research was supported by the Alberta Scholarship Programs (to C.S.S.), the Natural Sciences and Engineering Research Council of Canada (NSERC), the Canada Research Chair in Bioorganic and Medicinal Chemistry, the Alberta Heritage Foundation for Medical Research (AHFMR), and the United States Department of Agriculture—National Integrated Food Safety Initiative (USDA-NIFSI) Grant no. 2008-51110-0688.

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Graphical Abstract

Einblick ins Peptid: Für das antimikrobielle Peptid Thurincin H wurde eine Modifizierung nach der Translation vermutet. Eine MS/MS-Sequenzierung identifizierte nun die modifizierten Reste, und NMR-spektroskopische Studien in Lösung ergaben eine 3D-Struktur für Thurincin H, in der vier S-C_α-Thioether-Brücken auftreten (gelb im Bild). Diese Struktur könnte auch für einige andere Bacteriocine mit identischer Masse bedeutsam sein.

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Volume123, Issue37

September 5, 2011

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The 3D Solution Structure of Thurincin H, a Bacteriocin with Four Sulfur to α-Carbon Crosslinks^†

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The 3D Solution Structure of Thurincin H, a Bacteriocin with Four Sulfur to α-Carbon Crosslinks†

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The 3D Solution Structure of Thurincin H, a Bacteriocin with Four Sulfur to α-Carbon Crosslinks^†