The Cofactor of the Iron–Sulfur Cluster Free Hydrogenase Hmd: Structure of the Light-Inactivation Product†
Seigo Shima Dr.
Max Planck Institute for Terrestrial Microbiology, Karl-von-Frisch-Strasse, 35043 Marburg, Germany, Fax: (+49) 6421-178-209
These authors contributed equally to this work.
Search for more papers by this authorErica J. Lyon Dr.
Max Planck Institute for Terrestrial Microbiology, Karl-von-Frisch-Strasse, 35043 Marburg, Germany, Fax: (+49) 6421-178-209
These authors contributed equally to this work.
Search for more papers by this authorMelanie Sordel-Klippert
Max Planck Institute for Terrestrial Microbiology, Karl-von-Frisch-Strasse, 35043 Marburg, Germany, Fax: (+49) 6421-178-209
Search for more papers by this authorManuela Kauß
Max Planck Institute for Terrestrial Microbiology, Karl-von-Frisch-Strasse, 35043 Marburg, Germany, Fax: (+49) 6421-178-209
Search for more papers by this authorJörg Kahnt
Max Planck Institute for Terrestrial Microbiology, Karl-von-Frisch-Strasse, 35043 Marburg, Germany, Fax: (+49) 6421-178-209
Search for more papers by this authorRudolf K. Thauer Prof. Dr.
Max Planck Institute for Terrestrial Microbiology, Karl-von-Frisch-Strasse, 35043 Marburg, Germany, Fax: (+49) 6421-178-209
Search for more papers by this authorKlaus Steinbach Dr.
Fachbereich Chemie der Philipps Universität Marburg, Hans-Meerwein-Strasse, 35032 Marburg, Germany
Search for more papers by this authorXiulan Xie Dr.
Fachbereich Chemie der Philipps Universität Marburg, Hans-Meerwein-Strasse, 35032 Marburg, Germany
Search for more papers by this authorLaurent Verdier Dr.
Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Göttingen, Germany, Fax: (+49) 551-201-1320
Search for more papers by this authorChristian Griesinger Prof. Dr.
Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Göttingen, Germany, Fax: (+49) 551-201-1320
Search for more papers by this authorSeigo Shima Dr.
Max Planck Institute for Terrestrial Microbiology, Karl-von-Frisch-Strasse, 35043 Marburg, Germany, Fax: (+49) 6421-178-209
These authors contributed equally to this work.
Search for more papers by this authorErica J. Lyon Dr.
Max Planck Institute for Terrestrial Microbiology, Karl-von-Frisch-Strasse, 35043 Marburg, Germany, Fax: (+49) 6421-178-209
These authors contributed equally to this work.
Search for more papers by this authorMelanie Sordel-Klippert
Max Planck Institute for Terrestrial Microbiology, Karl-von-Frisch-Strasse, 35043 Marburg, Germany, Fax: (+49) 6421-178-209
Search for more papers by this authorManuela Kauß
Max Planck Institute for Terrestrial Microbiology, Karl-von-Frisch-Strasse, 35043 Marburg, Germany, Fax: (+49) 6421-178-209
Search for more papers by this authorJörg Kahnt
Max Planck Institute for Terrestrial Microbiology, Karl-von-Frisch-Strasse, 35043 Marburg, Germany, Fax: (+49) 6421-178-209
Search for more papers by this authorRudolf K. Thauer Prof. Dr.
Max Planck Institute for Terrestrial Microbiology, Karl-von-Frisch-Strasse, 35043 Marburg, Germany, Fax: (+49) 6421-178-209
Search for more papers by this authorKlaus Steinbach Dr.
Fachbereich Chemie der Philipps Universität Marburg, Hans-Meerwein-Strasse, 35032 Marburg, Germany
Search for more papers by this authorXiulan Xie Dr.
Fachbereich Chemie der Philipps Universität Marburg, Hans-Meerwein-Strasse, 35032 Marburg, Germany
Search for more papers by this authorLaurent Verdier Dr.
Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Göttingen, Germany, Fax: (+49) 551-201-1320
Search for more papers by this authorChristian Griesinger Prof. Dr.
Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Göttingen, Germany, Fax: (+49) 551-201-1320
Search for more papers by this authorThe authors thank Prof. Dr. Stefan Berger (Universität Leipzig) for the determination of the high-resolution mass data, Dr. Stefan Bartoschek and Dr. Peter Grice (University of Cambridge, UK) for recording the broadband 13C NMR spectrum on a 13C detection cryoprobe, Prof. Dr. Thomas Carell (Universität Marburg) for helpful discussions, and Dr. Antonio Pierik for assistance with the fluorescence measurements. L.V. was funded by the European research network HPRN CT 2000 00092.
Graphical Abstract
Eine Kombination von NMR-Spektroskopie und Massenspektrometrie lieferte die entscheidenden Informationen bei der Bestimmung der Struktur einer relativ stabilen, durch Licht desaktivierten Form des Cofaktors der Hydrogenase Hmd (siehe Schema). Den Untersuchungen zufolge ist ein Pyridon-Chromophor über eine Phosphatgruppe an GMP gebunden.
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