Genotypic analysis using a Y-chromosome-specific probe following bone marrow transplantation
Corresponding Author
Dr. Hiroko Morisaki
Department of Pathological Pharmacology, Institute of Medical Science, University of Tokyo, Tokyo, Japan
Department of Pathological Pharmacology, Institute of Medical Science, University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, Tokyo 108, JapanSearch for more papers by this authorTakayuki Morisaki
Department of Pathological Pharmacology, Institute of Medical Science, University of Tokyo, Tokyo, Japan
Search for more papers by this authorYutaka Nakahori
Department of Congenital Abnormalities Research, National Children's Medical Research Center, Tokyo, Japan
Search for more papers by this authorHiromi Ogura
Department of Pathological Pharmacology, Institute of Medical Science, University of Tokyo, Tokyo, Japan
Search for more papers by this authorHitoshi Kanno
Department of Pathological Pharmacology, Institute of Medical Science, University of Tokyo, Tokyo, Japan
Search for more papers by this authorKenzaburo Tani
Department of Pathological Pharmacology, Institute of Medical Science, University of Tokyo, Tokyo, Japan
Search for more papers by this authorHideki Kodo
Department of Pathological Pharmacology, Institute of Medical Science, University of Tokyo, Tokyo, Japan
Search for more papers by this authorHisaichi Fujii
Department of Pathological Pharmacology, Institute of Medical Science, University of Tokyo, Tokyo, Japan
Search for more papers by this authorShigetaka Asano
Department of Pathological Pharmacology, Institute of Medical Science, University of Tokyo, Tokyo, Japan
Search for more papers by this authorShiro Miwa
Department of Pathological Pharmacology, Institute of Medical Science, University of Tokyo, Tokyo, Japan
Search for more papers by this authorCorresponding Author
Dr. Hiroko Morisaki
Department of Pathological Pharmacology, Institute of Medical Science, University of Tokyo, Tokyo, Japan
Department of Pathological Pharmacology, Institute of Medical Science, University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, Tokyo 108, JapanSearch for more papers by this authorTakayuki Morisaki
Department of Pathological Pharmacology, Institute of Medical Science, University of Tokyo, Tokyo, Japan
Search for more papers by this authorYutaka Nakahori
Department of Congenital Abnormalities Research, National Children's Medical Research Center, Tokyo, Japan
Search for more papers by this authorHiromi Ogura
Department of Pathological Pharmacology, Institute of Medical Science, University of Tokyo, Tokyo, Japan
Search for more papers by this authorHitoshi Kanno
Department of Pathological Pharmacology, Institute of Medical Science, University of Tokyo, Tokyo, Japan
Search for more papers by this authorKenzaburo Tani
Department of Pathological Pharmacology, Institute of Medical Science, University of Tokyo, Tokyo, Japan
Search for more papers by this authorHideki Kodo
Department of Pathological Pharmacology, Institute of Medical Science, University of Tokyo, Tokyo, Japan
Search for more papers by this authorHisaichi Fujii
Department of Pathological Pharmacology, Institute of Medical Science, University of Tokyo, Tokyo, Japan
Search for more papers by this authorShigetaka Asano
Department of Pathological Pharmacology, Institute of Medical Science, University of Tokyo, Tokyo, Japan
Search for more papers by this authorShiro Miwa
Department of Pathological Pharmacology, Institute of Medical Science, University of Tokyo, Tokyo, Japan
Search for more papers by this authorAbstract
To monitor successful engraftment after bone marrow transplantation, we performed Southern hybridization analysis or dot blot analysis of DNA in a set of sex-mismatched cases using a Y-chromosome-specific DNA probe (pHY10). This method was extremely sensitive and rapid for checking which cells contain the Y-chromosome. Using this probe, analysis of cells from peripheral blood and bone marrow after transplantation demonstrated the usefulness of confirming engraftment of donor cells and of detecting mixed lymphohematopoietic chimerism.
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