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Enzyme Immobilization: Overcoming the Pitfalls of Cytochrome P450 Immobilization through the Use of Fusogenic Liposomes (Adv. Biosys. 1/2019)
First published: 11 January 2019
Graphical Abstract
Immobilization of drug metabolizing enzymes on solid supports improves prediction of drug-drug interactions by enabling enzyme inhibition assays under flow conditions. In article number 1800245, Tiina M. Sikanen and co-workers use fusogenic liposomes for biotinylation of lipid bilayers to facilitate immobilization of the membrane-bound cytochrome P450s on avidin-functionalized microfluidic devices. Preservation of the full complexity of the lipid environment ensures that the authentic enzyme kinetic parameters can be maintained.
