Chapter 7
FISH
Graeme N. Forrest,
Jwan Mohammadi,
Shahrzad Mohammadi,
Graeme N. Forrest
Portland Veterans Affairs Medical Center and Oregon Health and Science University, Department of Infectious Diseases, 3710 SW US Veterans Hospital Rd, P3-ID, Portland, OR 97239, USA
Search for more papers by this authorJwan Mohammadi
Oregon Health and Science University, Department of Infectious Diseases, 3181 SW Sam Jackson Park Rd, L-457, Portland, OR 97239, USA
Search for more papers by this authorShahrzad Mohammadi
Portland Veterans Affairs Medical Center and Oregon Health and Science University, Department of Infectious Diseases, 3710 SW US Veterans Hospital Rd, P3-ID, Portland, OR 97239, USA
Search for more papers by this authorGraeme N. Forrest,
Jwan Mohammadi,
Shahrzad Mohammadi,
Graeme N. Forrest
Portland Veterans Affairs Medical Center and Oregon Health and Science University, Department of Infectious Diseases, 3710 SW US Veterans Hospital Rd, P3-ID, Portland, OR 97239, USA
Search for more papers by this authorJwan Mohammadi
Oregon Health and Science University, Department of Infectious Diseases, 3181 SW Sam Jackson Park Rd, L-457, Portland, OR 97239, USA
Search for more papers by this authorShahrzad Mohammadi
Portland Veterans Affairs Medical Center and Oregon Health and Science University, Department of Infectious Diseases, 3710 SW US Veterans Hospital Rd, P3-ID, Portland, OR 97239, USA
Search for more papers by this authorBook Editor(s):Jürgen Popp,
Michael Bauer,
Jürgen Popp
Prof. Dr.
Friedrich-Schiller University Jena and Abbe Center of Photonics, Institute of Physical Chemistry, Helmholtzweg 4, 07743 Jena, Germany
Leibniz Institute of Photonic Technology, Albert-Einstein-Straße 9, 07745 Jena, Germany
Search for more papers by this authorMichael Bauer
Prof. Dr.
Jena University Hospital, Department of Anesthesiology and Intensive Care Medicine, Germany
Center for Sepsis Control and Care (CSCC), Erlanger Allee 101, 07747 Jena, Germany
Search for more papers by this authorFirst published: 27 February 2015
Summary
Fluorescence in situ hybridization (FISH) probes were first developed to locate the positions of specific DNA sequences on chromosomes. This chapter presents the current FISH tests that are available, their future directions, and their role and impact in clinical practice and patient care. Despite FISH tests being commercially available for almost a decade now, there remains very little prospective clinical data on the impact of these tests in the literature. There are currently two commercially available FISH techniques available for microbiology laboratories namely peptide nucleic acid fluorescence in situ hybridization (PNA FISH) and molecular beacons-based (bbFISH). There is now over a decade's clinical data on the use of PNA FISH in the clinical setting with different pathogens and countries. Most of the data is with blood stream infections, but increasingly there is new data on its use from other sites of infection.
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