Guidelines for the Functional Analysis of Engineered and Mutant Enzymes

Dale E. Edmondson

Departments of Biochemistry and Chemistry, Emory University, Atlanta, GA 30322-4098, USA

Giovanni Gadda

Departments of Chemistry and Biology, Georgia State University, The Center for Biotechnology and Drug Design, Atlanta, GA 30302-4098, USA

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Dale E. Edmondson,

Dale E. Edmondson

Departments of Biochemistry and Chemistry, Emory University, Atlanta, GA 30322-4098, USA

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Giovanni Gadda,

Giovanni Gadda

Departments of Chemistry and Biology, Georgia State University, The Center for Biotechnology and Drug Design, Atlanta, GA 30302-4098, USA

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Book Editor(s):Prof. Dr. Stefan Lutz,

Prof. Dr. Stefan Lutz

Department of Chemistry, Emory University, 1515 Dickey Drive, Atlanta, GA 30322, USA

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Prof. Dr. Uwe T. Bornscheuer,

Prof. Dr. Uwe T. Bornscheuer

Department of Biotechnology and Enzyme Catalysis, Institute of Biochemistry, Greifswald University, Felix-Hausdorff-Str. 4, 17487 Greifswald, Germany

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First published: 12 November 2008

https://doi.org/10.1002/9783527634026.ch1

Summary

This chapter contains sections titled:

Introduction
Steady-State Kinetics
Enzyme Assays and the Acquisition of Initial Velocity Data
Steady-State Kinetic Parameters and Their Interpretation
Concluding Remarks
References

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Protein Engineering Handbook, 1 & Volume 2