Depletion of multiple high-abundance proteins improves protein profiling capacities of human serum and plasma†
Originally published in Proteomics 2005, 13, 3292–3303
Summary
This chapter contains sections titled:
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Introduction
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Materials and methods
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Serum/plasma collection
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MARS
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Multiple affinity removal spin cartridge
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Microscale solution IEF (MicroSol IEF) (ZOOMTM-IEF) fractionation
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2-DE
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LC-MS/MS
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Results
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Depletion of major proteins to enhance detection of lower abundance proteins
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Evaluation of high-abundance protein removal using 2-DE
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Specificity of major protein depletion
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Impact of Top-6 protein depletion on detection of lower abundance proteins using 2-D gels
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Combining Top-6 protein depletion with microSol IEF prefractionation and narrow pH range gels
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Analysis of Top-6 depleted serum and plasma using protein array pixelation
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Discussion
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References
