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Citrus
Part 5. Transgenic Tropical and Subtropical Fruits and Nuts
Leandro Peña,
Magdalena Cervera,
Carmen Fagoaga,
Juan Romero,
Alida Ballester,
Nuria Soler,
Elsa Pons,
Ana Rodríguez,
Josep Peris,
José Juárez,
Luis Navarro,
Leandro Peña
Instituto Valenciano de Investigaciones Agrarias (IVIA), Centro de Protección Vegetal y Biotecnología, Moncada, Spain
Search for more papers by this authorMagdalena Cervera
Instituto Valenciano de Investigaciones Agrarias (IVIA), Centro de Protección Vegetal y Biotecnología, Moncada, Spain
Search for more papers by this authorCarmen Fagoaga
Instituto Valenciano de Investigaciones Agrarias (IVIA), Centro de Protección Vegetal y Biotecnología, Moncada, Spain
Search for more papers by this authorJuan Romero
Instituto Valenciano de Investigaciones Agrarias (IVIA), Centro de Protección Vegetal y Biotecnología, Moncada, Spain
Search for more papers by this authorAlida Ballester
Instituto Valenciano de Investigaciones Agrarias (IVIA), Centro de Protección Vegetal y Biotecnología, Moncada, Spain
Search for more papers by this authorNuria Soler
Instituto Valenciano de Investigaciones Agrarias (IVIA), Centro de Protección Vegetal y Biotecnología, Moncada, Spain
Search for more papers by this authorElsa Pons
Instituto Valenciano de Investigaciones Agrarias (IVIA), Centro de Protección Vegetal y Biotecnología, Moncada, Spain
Search for more papers by this authorAna Rodríguez
Instituto Valenciano de Investigaciones Agrarias (IVIA), Centro de Protección Vegetal y Biotecnología, Moncada, Spain
Search for more papers by this authorJosep Peris
Instituto Valenciano de Investigaciones Agrarias (IVIA), Centro de Protección Vegetal y Biotecnología, Moncada, Spain
Search for more papers by this authorJosé Juárez
Instituto Valenciano de Investigaciones Agrarias (IVIA), Centro de Protección Vegetal y Biotecnología, Moncada, Spain
Search for more papers by this authorLuis Navarro
Instituto Valenciano de Investigaciones Agrarias (IVIA), Centro de Protección Vegetal y Biotecnología, Moncada, Spain
Search for more papers by this authorLeandro Peña,
Magdalena Cervera,
Carmen Fagoaga,
Juan Romero,
Alida Ballester,
Nuria Soler,
Elsa Pons,
Ana Rodríguez,
Josep Peris,
José Juárez,
Luis Navarro,
Leandro Peña
Instituto Valenciano de Investigaciones Agrarias (IVIA), Centro de Protección Vegetal y Biotecnología, Moncada, Spain
Search for more papers by this authorMagdalena Cervera
Instituto Valenciano de Investigaciones Agrarias (IVIA), Centro de Protección Vegetal y Biotecnología, Moncada, Spain
Search for more papers by this authorCarmen Fagoaga
Instituto Valenciano de Investigaciones Agrarias (IVIA), Centro de Protección Vegetal y Biotecnología, Moncada, Spain
Search for more papers by this authorJuan Romero
Instituto Valenciano de Investigaciones Agrarias (IVIA), Centro de Protección Vegetal y Biotecnología, Moncada, Spain
Search for more papers by this authorAlida Ballester
Instituto Valenciano de Investigaciones Agrarias (IVIA), Centro de Protección Vegetal y Biotecnología, Moncada, Spain
Search for more papers by this authorNuria Soler
Instituto Valenciano de Investigaciones Agrarias (IVIA), Centro de Protección Vegetal y Biotecnología, Moncada, Spain
Search for more papers by this authorElsa Pons
Instituto Valenciano de Investigaciones Agrarias (IVIA), Centro de Protección Vegetal y Biotecnología, Moncada, Spain
Search for more papers by this authorAna Rodríguez
Instituto Valenciano de Investigaciones Agrarias (IVIA), Centro de Protección Vegetal y Biotecnología, Moncada, Spain
Search for more papers by this authorJosep Peris
Instituto Valenciano de Investigaciones Agrarias (IVIA), Centro de Protección Vegetal y Biotecnología, Moncada, Spain
Search for more papers by this authorJosé Juárez
Instituto Valenciano de Investigaciones Agrarias (IVIA), Centro de Protección Vegetal y Biotecnología, Moncada, Spain
Search for more papers by this authorLuis Navarro
Instituto Valenciano de Investigaciones Agrarias (IVIA), Centro de Protección Vegetal y Biotecnología, Moncada, Spain
Search for more papers by this authorAbstract
Citrus is the most important fruit tree crop in the world, with a production of more than 100 million tons annually. The area of origin of Citrus is believed to be southeastern Asia, where its domestication started. It has become clear that only citron, mandarin, and pummelo are true species within genus Citrus, being other important Citrus types, as sweet orange, sour orange, lemon, lime, grapefruit and other mandarins originated from hybridization between these ancestral species. In spite of the many efforts put in classical breeding programs in the last 100 years, current citrus industry relies on various groups of varieties that are grafted onto rootstocks adapted to different abiotic and biotic stresses. Most of these genotypes have been generated by chance, mostly as budsports but also as natural hybrids or seedlings selected by men in the wild or in orchards. Citrus breeding is complicated due to its complex reproductive biology. In this context, genetic transformation offers an important alternative for the genetic improvement of citrus. Moreover, it is probably the most efficient approach to make reverse genetics in citrus to investigate gene function and thus to gain better understanding in metabolic processes and plant-pathogen-environment interactions.
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Further Reading
- Chalfie, M., Tu, Y., Euskirchen, G., Ward, W.W. and Prasher, D.C. (1994) Green fluorescent protein as a marker for gene expression. Science 263, 663–664.
- Hood, E.E., Gelvin, S.B., Melchers, L.S. and Hoekema, A. (1993) New Agrobacterium helper plasmids for gene transfer to plants. Transgenic Research 2, 208–218.
- Jefferson, R.A., Kavanagh, T.A. and Bevan, M.W. (1987) GUS fusions: β-glucuronidase as a sensitive and versatile gene fusion marker in higher plants. EMBO Journal 6, 3901–3907.
- Sanford, J.C. and Johnston, S.A. (1985) The concept of parasite-derived resistance. Deriving resistance genes from the parasite's own genome. Journal of Theoretical Biology 113, 395–405.
