Chapter 2
Cellular Requirements and Preparation for Bioprinting
Shalini Dasgupta,
Vriti Sharma,
Ananya Barui,
Shalini Dasgupta
Centre for Healthcare Science and Technology, Indian Institute of Engineering Science and Technology, Shibpur, India
Search for more papers by this authorVriti Sharma
Centre for Healthcare Science and Technology, Indian Institute of Engineering Science and Technology, Shibpur, India
Search for more papers by this authorAnanya Barui
Centre for Healthcare Science and Technology, Indian Institute of Engineering Science and Technology, Shibpur, India
Search for more papers by this authorShalini Dasgupta,
Vriti Sharma,
Ananya Barui,
Shalini Dasgupta
Centre for Healthcare Science and Technology, Indian Institute of Engineering Science and Technology, Shibpur, India
Search for more papers by this authorVriti Sharma
Centre for Healthcare Science and Technology, Indian Institute of Engineering Science and Technology, Shibpur, India
Search for more papers by this authorAnanya Barui
Centre for Healthcare Science and Technology, Indian Institute of Engineering Science and Technology, Shibpur, India
Search for more papers by this authorBook Editor(s):Prosenjit Saha, Sabu Thomas, Jinku Kim, Manojit Ghosh,
Manojit Ghosh
Indian Institute of Engineering Science and Technology (IIEST), Howrah, India
Search for more papers by this authorFirst published: 05 July 2024
Summary
3D bioprinting has been an upcoming technique supporting the development of artificial organs in the twenty-first century. While 3D printing has been around for a few decades and has been utilized in construction, robotics, automobile industry etc, bioprinting is a much more specialized niche developed only for healthcare. It comprises of biocompatible material with the physical properties required to maintain fidelity for 3D printed shapes, combined with live cells mixed with the ink and being printed in real time. It involves an extensive understanding of organ dynamics, computational modelling, knowledge of material science and cell biology. Especially, as living cells are now involved in the development of tissue-engineered constructs for organ regeneration. Cell-embedded bioink requires more comprehensive handling and sterile printing parameters with strict limitations upon design, viscosity and pressure in order to prevent tissue damage. In this book chapter, we will discuss the potential of 3D bioprinting for tissue engineering, types of bioink and bioprinters and the different cellular requirements associated with fabrication of a satisfactory 3d printed organ with the requisite features required for growth, differentiation and maturation.
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