Light Microscopy and CSLM Techniques, Principles and Applications

Light microscopy and confocal scanning laser microscopy are widely used imaging techniques to generate structural insights in the complex interplay of components in food and processed food. Focusing on the application of optical microscopy (light and confocal scanning laser microscopy) to dairy products, this chapter briefly discusses the history of the technique and evolution of food microscopy. It describes and discusses the differences in imaging modes as well as the methodology to obtain a specimen or sub-sample that represents the original and undisturbed microstructure. Staining and specific labeling procedures are discussed for both bright field and confocal laser scanning microscopy. It reflects on applications for dynamic imaging using temperature control and shear stages to study microstructural changes during processing.

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Microstructure of Dairy Products