Chapter 37

Changes in Active Site Hydrogen Bonding upon Formation of the Electronically Excited State of Photoactive Yellow Protein

Wouter D. Hoff

Wouter D. Hoff

Department of Microbiology and Molecular Genetics, Oklahoma State University, Stillwater, OK 74078, USA

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Zhouyang Kang

Zhouyang Kang

Department of Physics, Oklahoma State University, Stillwater, Oklahoma 74078, USA

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Masato Kumauchi

Masato Kumauchi

Department of Microbiology and Molecular Genetics, Oklahoma State University, Stillwater, OK 74078, USA

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Aihua Xie

Aihua Xie

Department of Physics, Oklahoma State University, Stillwater, Oklahoma 74078, USA

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First published: 22 October 2010

Summary

This chapter contains sections titled:

  • Central Importance of Light in Biology

  • Possible Importance of Excited State Hydrogen Bonding in Photoreceptors

  • Introduction to Photoactive Yellow Protein

  • Hydrogen Bonding in the Initial State of PYP

  • Assignment of Vibrational Modes in PYP

  • Identification of Vibrational Structural Markers

  • Changes in Hydrogen Bonding During the Initial Stages of the PYP Photocycle

  • Sub-Picosecond Time-Resolved Transient Spectroscopy of PYP

  • Changes in Active Site Hydrogen Bonding upon the Formation of the S1 State of PYP

  • Excited State Proton Transfer in the Y42F Mutant of PYP

  • Acknowledgements

  • References

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