Fibrous Proteins from Recombinant Microorganisms

Part 8. Polyamides and Complex Proteinaceous Materials
Stephen R. Fahnestock

Stephen R. Fahnestock

E. I. DuPont de Nemours & Co., Wilmington, DE, USA, 19880-0328

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First published: 15 January 2005

Abstract

  • Introduction
    • Sequence-Defined Polymers
    • Microbial Production
  • Historical Outline
    • Expression of Natural Silk Genes in Microbes
    • Design of Synthetic Genes
  • Silk-Like Proteins
    • Summary of Strategy
    • Commercial Applications
  • Spider Silk Analog Proteins
    • Major Dragline Protein Gene Construction
    • Gene Expression in E. coli
    • Second Dragline Protein
    • Stability of Synthetic Genes in E. coli
    • Truncated Protein Synthesis
    • Dragline Silk Protein in Yeast
    • Protein Secretion
    • Properties of DP-1B and DP-2A Proteins
      • Solution Properties
      • Fiber Formation
    • Other Spider Silk Analogs
      • Single-Molecule Structural Studies
      • Silk Protein Engineered for Processing Efficiency
      • Flagelliform Silk Analog Protein
  • Other Designed Protein Polymers
    • A Designed Self-Assembling Fibrillar Protein
    • Elastin-Like Proteins
    • Collagen Analogs
  • Cost of Production
  • Patents
  • Future Prospects

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