Volume 84, Issue 6 pp. 1347-1354
Research Article

Hydrolysis of tRNAPhe on Suspensions of Amino Acids

Kui Gao

Kui Gao

The Salk Institute for Biological Studies, Post Office Box 85800, San Diego, CA 92186-5800, USA (Tel: (858) 453-4100, ext. 1322; Fax: (858) 558-7359)

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Leslie E. Orgel

Leslie E. Orgel

The Salk Institute for Biological Studies, Post Office Box 85800, San Diego, CA 92186-5800, USA (Tel: (858) 453-4100, ext. 1322; Fax: (858) 558-7359)

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Abstract

RNA is adsorbed strongly on suspensions of many moderately soluble organic solids. In some cases, the hydrolysis of tRNAPhe is greatly accelerated by adsorption, and the major sites of hydrolysis are changed from those that are important in homogeneous solution. Here we show that the hydrolysis is greatly accelerated by suspensions of aspartic acid and β-glutamic acid but not by suspensions of α-glutamic acid, asparagine, or glutamine.

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