Communication

Catalyst for DNA Ligation: Towards a Two-Stage Replication Cycle

Jingdong Ye

Departments of Chemistry and Biology, Emerson Hall Emory University, Atlanta, GA 30322 (USA) Department of Chemistry University of Chicago Chicago, IL 60637 Fax: (+1) 404-727-9348

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Yahaloma Gat,

Yahaloma Gat

Departments of Chemistry and Biology, Emerson Hall Emory University, Atlanta, GA 30322 (USA) Department of Chemistry University of Chicago Chicago, IL 60637 Fax: (+1) 404-727-9348

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David G. Lynn,

David G. Lynn

[email protected]

Departments of Chemistry and Biology, Emerson Hall Emory University, Atlanta, GA 30322 (USA) Department of Chemistry University of Chicago Chicago, IL 60637 Fax: (+1) 404-727-9348

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Jingdong Ye,

Jingdong Ye

Departments of Chemistry and Biology, Emerson Hall Emory University, Atlanta, GA 30322 (USA) Department of Chemistry University of Chicago Chicago, IL 60637 Fax: (+1) 404-727-9348

Search for more papers by this author

Yahaloma Gat,

Yahaloma Gat

Departments of Chemistry and Biology, Emerson Hall Emory University, Atlanta, GA 30322 (USA) Department of Chemistry University of Chicago Chicago, IL 60637 Fax: (+1) 404-727-9348

Search for more papers by this author

David G. Lynn,

David G. Lynn

[email protected]

Departments of Chemistry and Biology, Emerson Hall Emory University, Atlanta, GA 30322 (USA) Department of Chemistry University of Chicago Chicago, IL 60637 Fax: (+1) 404-727-9348

Search for more papers by this author

First published: 13 October 2000

https://doi.org/10.1002/1521-3773(20001016)39:20<3641::AID-ANIE3641>3.0.CO;2-J

Citations: 36

We are grateful to Paul Gardner for assistance with solid-phase synthesis and the NIH (R21 RR12723) for support.

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Abstract

Modification of the DNA backbone by replacement of a single phosphate with an alkylamine is shown to generate a selective ligation catalyst. Related backbone modifications can be chemically read from a complementary DNA sequence. With this demonstration, it now appears possible to generate a two-stage replication cycle (see scheme), a simplified version of the biological three-stage DNA–RNA–protein cycle necessary for biological replication.

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Citing Literature

Volume39, Issue20

October 16, 2000

Pages 3641-3643

Catalyst for DNA Ligation: Towards a Two-Stage Replication Cycle

Abstract

References

Citing Literature

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