Genotoxicity of instant coffee and of some phenolic compounds present in coffee upon nitrosation
Maria Paula Duarte
Department of Genetics, Faculty of Medical Sciences, New University of Lisbon, Lisbon, Portugal
Faculty of Sciences and Technology, New University of Lisbon, Lisbon, Portugal
Search for more papers by this authorAntónio Laires
Department of Genetics, Faculty of Medical Sciences, New University of Lisbon, Lisbon, Portugal
Faculty of Sciences and Technology, New University of Lisbon, Lisbon, Portugal
Search for more papers by this authorJorge Gaspar
Department of Genetics, Faculty of Medical Sciences, New University of Lisbon, Lisbon, Portugal
U.L.H.T., Campo Grande, Lisbon, Portugal
Search for more papers by this authorJosé Santos Oliveira
Faculty of Sciences and Technology, New University of Lisbon, Lisbon, Portugal
Search for more papers by this authorCorresponding Author
José Rueff
Department of Genetics, Faculty of Medical Sciences, New University of Lisbon, Lisbon, Portugal
Department of Genetics, Faculty of Medical Sciences, New University of Lisbon, R. da Junqueira 96, P-1349-008 Lisbon, PortugalSearch for more papers by this authorMaria Paula Duarte
Department of Genetics, Faculty of Medical Sciences, New University of Lisbon, Lisbon, Portugal
Faculty of Sciences and Technology, New University of Lisbon, Lisbon, Portugal
Search for more papers by this authorAntónio Laires
Department of Genetics, Faculty of Medical Sciences, New University of Lisbon, Lisbon, Portugal
Faculty of Sciences and Technology, New University of Lisbon, Lisbon, Portugal
Search for more papers by this authorJorge Gaspar
Department of Genetics, Faculty of Medical Sciences, New University of Lisbon, Lisbon, Portugal
U.L.H.T., Campo Grande, Lisbon, Portugal
Search for more papers by this authorJosé Santos Oliveira
Faculty of Sciences and Technology, New University of Lisbon, Lisbon, Portugal
Search for more papers by this authorCorresponding Author
José Rueff
Department of Genetics, Faculty of Medical Sciences, New University of Lisbon, Lisbon, Portugal
Department of Genetics, Faculty of Medical Sciences, New University of Lisbon, R. da Junqueira 96, P-1349-008 Lisbon, PortugalSearch for more papers by this authorAbstract
Instant coffee exhibits genotoxic activity upon nitrosation at acidic pH values in the Ames tester strain TA100. Using adsorption chromatography (Amberlit XAD-2) it was observed that the major fraction of molecules responsible for the genotoxic activity upon nitrosation was not retained on this resin, suggesting that the polar molecules present in instant coffee could be responsible for the genotoxicity observed upon nitrosation. Some phenolic molecules present in instant coffee (catechol, caffeic acid, and chlorogenic acid) were also genotoxic upon nitrosation under the same experimental conditions. The concentrations of nitrosatable phenolic compounds in the studied coffee were determined by HPLC and their contributions to the total genotoxicity observed were studied. The results obtained suggest that besides phenolic compounds other molecules were also involved in the genotoxicity of this beverage upon nitrosation. Teratogenesis Carcinog. Mutagen. 20:241–249, 2000. © 2000 Wiley-Liss, Inc.
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