Liquid Chromatography, 4. Affinity Chromatography

Karin Cabrera

Karin Cabrera

Merck KGaA, Darmstadt, Germany

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Wolfgang Brünner

Wolfgang Brünner

Merck KGaA, Darmstadt, Germany

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Egbert Müller

Egbert Müller

Merck KGaA, Darmstadt, Germany

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First published: 15 October 2011

Abstract

The article contains sections titled:

1.

Introduction

2.

Preparation of an Affinity Matrix

2.1.

Selection of a Carrier

2.2.

Immobilization of the Ligand

3.

Ligands

3.1.

Group-Specific Ligands

3.1.1.

Low-Molecular Ligands

3.1.2.

Macromolecular Ligands

3.2.

Monospecific Ligands

4.

Elution

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