Biotechnology, 3. Cultivation of Microorganisms

Horst Werner Doelle

Horst Werner Doelle

University of Queensland, Department of Microbiology, St. Lucia, Queensland, Australia, 4067

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Armin Fiechter

Armin Fiechter

Eidgenössische Technische Hochschule, Institute of Biotechnology, Zürich, Switzerland

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Günther Schlegel

Günther Schlegel

University of Göttingen, Institute of Microbiology, Göttingen, Germany

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Sakayu Shimizu

Sakayu Shimizu

Kyoto University, Department of Agricultural Chemistry, Kyoto, Japan

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Roland Ulber

Roland Ulber

University of Kaiserslautern, Institute of Bioprocess Engineering, Germany

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Hideaki Yamada

Hideaki Yamada

Kyoto University, Department of Agricultural Chemistry, Kyoto, Japan

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First published: 15 July 2009

Abstract

The article contains sections titled:

1.

Introduction

2.

Isolation of Microorganisms

3.

Requirements for Growth

3.1.

Chemical Composition of Bacterial Cells

3.2.

Carbon and Energy Sources

3.3.

Accessory Nutrients

3.4.

Sulfur and Nitrogen

3.5.

Oxygen

3.6.

Complex Media

3.7.

Solid Media

3.8.

Hydrogen Ion Concentration

3.9.

Carbon Dioxide

3.10.

Aeration

3.11.

Anaerobic Techniques

3.12.

Media Preparation

4.

Sterilization

4.1.

Moist Heat

4.2.

Dry Heat

4.3.

Filtration

4.4.

Irradiation

4.5.

Chemical Means

5.

Types of Bioprocesses

5.1.

Surface Culture

5.2.

Submerged Culture

6.

Process Layout

6.1.

Reactors

6.2.

Containments for Anaerobic Processes

6.3.

Reactors for Aerobic Processes

6.4.

Inoculation

6.5.

Operation Modes

7.

Process and Product Overview

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