Biotechnology, 2. Metabolic Engineering

1.	Introduction
2.	Analysis of the Transcriptome, Proteome, and Metabolome
2.1.	Gene Expression Analysis using DNA Microarrays
2.2.	Fabrication of DNA microarrays
2.3.	Proteome Analysis using Protein Microarrays
2.4.	Metabolome Analysis and Metabolite flux analysis
3.	Design and Production of Genetically Optimized Strains for Production – In Vitro Mutagenesis
4.	Random Mutagenesis, Isolation and Selection of Mutants
5.	Types of Mutants and Selection Principles
5.1.	Auxotrophic Mutants
5.2.	Regulatory Mutants
5.3.	Other Selection Methods
5.4.	Targeted or Site-Directed Mutagenesis

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