Liquid Chromatography, 1. Fundamentals, History, Instrumentation, Materials

Pavel Jandera

Pavel Jandera

University of Pardubice, Pardubice, Czech Republic

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Günter Henze

Günter Henze

Institut für Anorganische und Analytische Chemie deA Technischen Universität, Clausthal, Germany

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First published: 15 October 2011

Abstract

The article contains sections titled:

1.

Introduction

1.1.

History

1.2.

Definition and Theoretical Background

2.

Equipment

2.1.

Solvent Reservoir, Filters, Degasser and Connecting Tubing

2.2.

Pumps

2.3.

Sample Introduction Units

2.4.

Column Hardware

2.5.

Detectors

2.5.1.

Photometric Detectors

2.5.2.

The Fluorescence Detector

2.5.3.

Refractive Index (RI) Detectors

2.5.4.

Light-Scattering Detectors

2.5.5.

Electrochemical Detectors

2.6.

Data Processing Systems

3.

Packing Materials and Stationary Phases for HPLC

3.1.

Types and Porosity of Column Packing Materials

3.2.

Particle Size, Hydrodynamic Aspects of Column Operation

3.3.

Superficially Porous Particles

3.4.

Monolithic Columns

3.5.

Stationary Phases for Liquid Chromatography

3.5.1.

Silica Gel

3.5.2.

Metal Oxides

3.5.3.

Chemically Bonded Phases

3.5.4.

Carbon Adsorbents

3.5.5.

Organic Polymer Adsorbents

3.5.6.

Characterization of HPLC Column Properties

4.

The Mobile Phase

4.1.

Purification and Pretreatment of LC Solvents

4.2.

Selection of Mobile Phase

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