Bioseparation

1.	Introduction
2.	Cell Disruption
3.	Solid–Liquid Separation
3.1.	Conventional Filtration
3.2.	Membrane Filtration
3.2.1.	Promotion of Turbulence
3.2.2.	Application of Electric Field
3.3.	Centrifugation
3.4.	Coagulation and Flocculation
4.	Product Isolation
4.1.	Extraction
4.2.	Adsorption Including Ion Exchange
4.2.1.	Affinity Adsorption
4.2.2.	Ion-Exchange Adsorption
4.2.3.	Quantitative Analysis
4.2.4.	Elution
4.3.	Precipitation
4.3.1.	Salting Out
4.3.2.	pH Adjustment
4.3.3.	Organic Solvents
4.3.4.	Temperature
4.3.5.	Polymers and Polyelectrolytes
4.3.6.	Affinity Precipitation
4.4.	Solubilization and Refolding
4.5.	Ultrafiltration
5.	Purification
5.1.	Chromatography
5.1.1.	Gel Filtration Chromatography
5.1.2.	Affinity Chromatography
5.1.3.	Reverse Phase and Hydrophobic Interaction Chromatography
5.1.4.	Quantitative Aspects
5.2.	Electrical Separations
5.2.1.	Electrophoresis
5.2.2.	Isoelectric Focusing and Isotachophoresis
5.2.3.	Electrodialysis
5.2.4.	Dielectrophoresis
5.3.	Magnetic Separations
5.4.	Membrane Chromatography
6.	Polishing
6.1.	Crystallization
6.2.	Drying and Freeze Drying
6.3.	Buffer Exchange
6.4.	Virus and Endotoxin Removal
6.5.	Removal of Aggregates
7.	New Bioseparation Strategies

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