Sensitive detection of transitional cell carcinoma of the bladder by microsatellite analysis of cells exfoliated in urine
Davide Seripa
Unità Patologia Molecolare e Terapia Genica, IRCCS H. “Casa Sollievo Sofferenza”, Opera Padre Pio da Pietrelcina, San Giovanni Rotondo, Italy
The first two authors contributed equally to this work.
Search for more papers by this authorPaola Parrella
Laboratory of Molecular Medicine and BioTechnology, Università Campus Bio-Medico, Rome, Italy
Department of Otolaryngology—Head and Neck Surgery, Division of Head and Neck Cancer Research, Johns Hopkins University School of Medicine, Baltimore, MD, USA
The first two authors contributed equally to this work.
Search for more papers by this authorMichele Gallucci
Department of Urology, Campus Bio-Medico University, Rome, Italy
Department of Urology, “Cristo Re” Hospital, Rome, Italy
Search for more papers by this authorCarolina Gravina
Unità Patologia Molecolare e Terapia Genica, IRCCS H. “Casa Sollievo Sofferenza”, Opera Padre Pio da Pietrelcina, San Giovanni Rotondo, Italy
Search for more papers by this authorSara Papa
Unità Patologia Molecolare e Terapia Genica, IRCCS H. “Casa Sollievo Sofferenza”, Opera Padre Pio da Pietrelcina, San Giovanni Rotondo, Italy
Search for more papers by this authorPasquale Fortunato
Department of Urology, “Cristo Re” Hospital, Rome, Italy
Search for more papers by this authorAntonio Alcini
Department of Urology, Campus Bio-Medico University, Rome, Italy
Search for more papers by this authorGerardo Flammia
Department of Urology, Campus Bio-Medico University, Rome, Italy
Search for more papers by this authorMarzia Lazzari
Department of Surgery, Università di Roma “Tor Vergata”, Rome, Italy
Search for more papers by this authorCorresponding Author
Vito M. Fazio
Unità Patologia Molecolare e Terapia Genica, IRCCS H. “Casa Sollievo Sofferenza”, Opera Padre Pio da Pietrelcina, San Giovanni Rotondo, Italy
Laboratory of Molecular Medicine and BioTechnology, Università Campus Bio-Medico, Rome, Italy
Fax: +39-882-410575 or +39-882-410794
Molecular Pathology and Gene Therapy Unit, IRCCS-II, “Casa Sollievo della Sofferenza,” Viale Cappuccini, I, San Giovanni Rotondo (FG), I-71013, ItalySearch for more papers by this authorDavide Seripa
Unità Patologia Molecolare e Terapia Genica, IRCCS H. “Casa Sollievo Sofferenza”, Opera Padre Pio da Pietrelcina, San Giovanni Rotondo, Italy
The first two authors contributed equally to this work.
Search for more papers by this authorPaola Parrella
Laboratory of Molecular Medicine and BioTechnology, Università Campus Bio-Medico, Rome, Italy
Department of Otolaryngology—Head and Neck Surgery, Division of Head and Neck Cancer Research, Johns Hopkins University School of Medicine, Baltimore, MD, USA
The first two authors contributed equally to this work.
Search for more papers by this authorMichele Gallucci
Department of Urology, Campus Bio-Medico University, Rome, Italy
Department of Urology, “Cristo Re” Hospital, Rome, Italy
Search for more papers by this authorCarolina Gravina
Unità Patologia Molecolare e Terapia Genica, IRCCS H. “Casa Sollievo Sofferenza”, Opera Padre Pio da Pietrelcina, San Giovanni Rotondo, Italy
Search for more papers by this authorSara Papa
Unità Patologia Molecolare e Terapia Genica, IRCCS H. “Casa Sollievo Sofferenza”, Opera Padre Pio da Pietrelcina, San Giovanni Rotondo, Italy
Search for more papers by this authorPasquale Fortunato
Department of Urology, “Cristo Re” Hospital, Rome, Italy
Search for more papers by this authorAntonio Alcini
Department of Urology, Campus Bio-Medico University, Rome, Italy
Search for more papers by this authorGerardo Flammia
Department of Urology, Campus Bio-Medico University, Rome, Italy
Search for more papers by this authorMarzia Lazzari
Department of Surgery, Università di Roma “Tor Vergata”, Rome, Italy
Search for more papers by this authorCorresponding Author
Vito M. Fazio
Unità Patologia Molecolare e Terapia Genica, IRCCS H. “Casa Sollievo Sofferenza”, Opera Padre Pio da Pietrelcina, San Giovanni Rotondo, Italy
Laboratory of Molecular Medicine and BioTechnology, Università Campus Bio-Medico, Rome, Italy
Fax: +39-882-410575 or +39-882-410794
Molecular Pathology and Gene Therapy Unit, IRCCS-II, “Casa Sollievo della Sofferenza,” Viale Cappuccini, I, San Giovanni Rotondo (FG), I-71013, ItalySearch for more papers by this authorAbstract
Transitional cell carcinoma (TCC) is the most common bladder tumor. Urine cytology can identify most high-grade tumors but sensitivity is lower if one includes lesions of all grades. Microsatellite marker alterations have been found in many tumor types including bladder cancer and have been used to detect cancer cells in body fluids including urine. The aim of our study is to further evaluate feasibility and sensitivity of microsatellite analysis to detect bladder cancer cells in urine. We studied 55 individuals: 21 with symptoms suggestive of bladder cancer, 23 patients with previous history of TCC and 11 healthy subjects. Genomic DNA was extracted from blood lymphocytes, urine sediment, bladder washings and tumor or normal bladder mucosa. Twenty highly informative microsatellite markers were analyzed for loss of heterozigosity (LOH) and microsatellite instability (MIN) by polymerase chain reaction. Microsatellite analysis of urine identified 33 of 34 (97%) patients with either primary or tumor recurrence, whereas urine cytology identified 27 of 34 (79%) patients (p = 0.0001). Detection of microsatellite abnormalities improved the sensitivity of detecting low-grade and/or stage bladder tumor: from 75–95% for grades G1–G2 and from 75–100% for pTis–pTa tumors. Bladder washings from 25 patients were also analyzed, and in all cases results were identical to those obtained from voided urine. None of the 16 patients without evidence of TCC showed LOH and/or MIN in urine samples or bladder washings. Interestingly, in a patient with persistent bladder mucosa abnormalities, microsatellite alterations were demonstrated 8 months before the histopathologic diagnosis of tumor recurrence. These results further indicate that microsatellite marker analysis is more sensitive than conventional urine cytology in detecting bladder cancer cells in urine and represents a potential clinical tool for monitoring patients with low-grade/stage TCC. © 2001 Wiley-Liss, Inc.
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