Volume 5, Issue 6 pp. 371-377
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Biological effects of rare earth protein complexes: Influence of lanthanide ions Eu3+, Tb3+ on secondary structure of calmodulins

Yuan-Yuan Song

Yuan-Yuan Song

Department of Chemistry, State Key Laboratory of Rare Earth Material Chemistry and Applications, Peking University–The University of Hong Kong Joint Laboratory in Rare Earth Materials and Bioinorganic Chemistry, Peking University, Beijing 100871, People's Republic of China

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Yi-Zhuang Xu

Yi-Zhuang Xu

Department of Chemistry, State Key Laboratory of Rare Earth Material Chemistry and Applications, Peking University–The University of Hong Kong Joint Laboratory in Rare Earth Materials and Bioinorganic Chemistry, Peking University, Beijing 100871, People's Republic of China

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Shi-Fu Weng

Shi-Fu Weng

Department of Chemistry, State Key Laboratory of Rare Earth Material Chemistry and Applications, Peking University–The University of Hong Kong Joint Laboratory in Rare Earth Materials and Bioinorganic Chemistry, Peking University, Beijing 100871, People's Republic of China

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Li-Bo Wang

Li-Bo Wang

Department of Chemistry, State Key Laboratory of Rare Earth Material Chemistry and Applications, Peking University–The University of Hong Kong Joint Laboratory in Rare Earth Materials and Bioinorganic Chemistry, Peking University, Beijing 100871, People's Republic of China

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Xiao-Feng Li

Xiao-Feng Li

Department of Chemistry, State Key Laboratory of Rare Earth Material Chemistry and Applications, Peking University–The University of Hong Kong Joint Laboratory in Rare Earth Materials and Bioinorganic Chemistry, Peking University, Beijing 100871, People's Republic of China

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Ting-Fang Zhang

Ting-Fang Zhang

Department of Chemistry, State Key Laboratory of Rare Earth Material Chemistry and Applications, Peking University–The University of Hong Kong Joint Laboratory in Rare Earth Materials and Bioinorganic Chemistry, Peking University, Beijing 100871, People's Republic of China

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Jin-Guang Wu

Corresponding Author

Jin-Guang Wu

Department of Chemistry, State Key Laboratory of Rare Earth Material Chemistry and Applications, Peking University–The University of Hong Kong Joint Laboratory in Rare Earth Materials and Bioinorganic Chemistry, Peking University, Beijing 100871, People's Republic of China

Department of Chemistry, State Key Laboratory of Rare Earth Material Chemistry and Applications, Peking University–The University of Hong Kong Joint Laboratory in Rare Earth Materials and Bioinorganic Chemistry, Peking University, Beijing 100871, People's Republic of ChinaSearch for more papers by this author

Abstract

The secondary structure of four kinds of calmodulins (CaMs; i.e., Brassica campestris pollen CaM, bovine brain CaM, earthworm calcium binding protein, and earthworm new calcium binding protein) in thin films are determined by the FTIR resolution enhanced technique and curve fitting. The variation in the secondary structure of CaM upon its binding with Ca2+, Eu3+, and Tb3+, the assay of phosphodiesterase enzyme, and sodium dodecyl sulfate-polyacrylamide gel electrophoresis are also investigated. The effect of lanthanide ions on the conformation of CaM are described. © 1999 John Wiley & Sons, Inc. Biospectroscopy 5: 371–377, 1999

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