Isolation, mapping, and functional analysis of a novel human cDNA (BNIP3L) encoding a protein homologous to human NIP3
Mieko Matsushima
Human Genome Center, Institute of Medical Science, University of Tokyo, Tokyo, Japan
Department of Gynecology and Obstetrics, Nagoya City University, Nagoya, Japan
Search for more papers by this authorTsutomu Fujiwara
Otsuka GEN Research Institute, Otsuka Pharmaceutical Co., Ltd., Tokushima, Japan
Search for more papers by this authorEi-ichi Takahashi
Otsuka GEN Research Institute, Otsuka Pharmaceutical Co., Ltd., Tokushima, Japan
Search for more papers by this authorTakeo Minaguchi
Human Genome Center, Institute of Medical Science, University of Tokyo, Tokyo, Japan
Search for more papers by this authorYutaka Eguchi
Department of Medical Genetics, Biomedical Research Center, Osaka University Medical School, Osaka, Japan
Search for more papers by this authorYoshihide Tsujimoto
Department of Medical Genetics, Biomedical Research Center, Osaka University Medical School, Osaka, Japan
Search for more papers by this authorKaoru Suzumori
Department of Gynecology and Obstetrics, Nagoya City University, Nagoya, Japan
Search for more papers by this authorCorresponding Author
Yusuke Nakamura
Human Genome Center, Institute of Medical Science, University of Tokyo, Tokyo, Japan
Laboratory of Molecular Medicine, Human Genome Center, Institute of Medical Science, University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, Tokyo 108, JapanSearch for more papers by this authorMieko Matsushima
Human Genome Center, Institute of Medical Science, University of Tokyo, Tokyo, Japan
Department of Gynecology and Obstetrics, Nagoya City University, Nagoya, Japan
Search for more papers by this authorTsutomu Fujiwara
Otsuka GEN Research Institute, Otsuka Pharmaceutical Co., Ltd., Tokushima, Japan
Search for more papers by this authorEi-ichi Takahashi
Otsuka GEN Research Institute, Otsuka Pharmaceutical Co., Ltd., Tokushima, Japan
Search for more papers by this authorTakeo Minaguchi
Human Genome Center, Institute of Medical Science, University of Tokyo, Tokyo, Japan
Search for more papers by this authorYutaka Eguchi
Department of Medical Genetics, Biomedical Research Center, Osaka University Medical School, Osaka, Japan
Search for more papers by this authorYoshihide Tsujimoto
Department of Medical Genetics, Biomedical Research Center, Osaka University Medical School, Osaka, Japan
Search for more papers by this authorKaoru Suzumori
Department of Gynecology and Obstetrics, Nagoya City University, Nagoya, Japan
Search for more papers by this authorCorresponding Author
Yusuke Nakamura
Human Genome Center, Institute of Medical Science, University of Tokyo, Tokyo, Japan
Laboratory of Molecular Medicine, Human Genome Center, Institute of Medical Science, University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, Tokyo 108, JapanSearch for more papers by this authorAbstract
We have isolated a novel cDNA that encodes a product showing significant sequence homology (56% identity) to human NIP3, a protein thought to interact with adenovirus E1B19kD and human BCL2 proteins. This cDNA contains an open reading frame of 657 nucleotides encoding a 219 amino acid polypeptide. The gene, designated BNIP3L, was expressed in all 16 normal human tissues examined; we mapped it to chromosome band 8p21 by fluorescence in situ hybridization. Introduction of the BNIP3L gene into six different cancer-cell lines caused significant growth suppression in each of them, while no such effect occurred when the antisense cDNA or the vector DNA was transfected, indicating that BNIP3L may function as a tumor suppressor. Genes Chromosomes Cancer 21:230–235, 1998. © 1998 Wiley-Liss, Inc.
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