Mutation detection by solid phase primer extension
Corresponding Author
John M. Shumaker
Department of Molecular and Human Genetics and Howard Hughes Medical Institute, Baylor College of Medicine, Houston, Texas 77030
Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, Texas 77030Search for more papers by this authorAndres Metspalu
Department of Molecular and Human Genetics and Howard Hughes Medical Institute, Baylor College of Medicine, Houston, Texas 77030
Tartu University, Tartu, Estonia Fax: 713-798-7383
Search for more papers by this authorC. Thomas Caskey
Department of Molecular and Human Genetics and Howard Hughes Medical Institute, Baylor College of Medicine, Houston, Texas 77030
Search for more papers by this authorCorresponding Author
John M. Shumaker
Department of Molecular and Human Genetics and Howard Hughes Medical Institute, Baylor College of Medicine, Houston, Texas 77030
Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, Texas 77030Search for more papers by this authorAndres Metspalu
Department of Molecular and Human Genetics and Howard Hughes Medical Institute, Baylor College of Medicine, Houston, Texas 77030
Tartu University, Tartu, Estonia Fax: 713-798-7383
Search for more papers by this authorC. Thomas Caskey
Department of Molecular and Human Genetics and Howard Hughes Medical Institute, Baylor College of Medicine, Houston, Texas 77030
Search for more papers by this authorAbstract
A mutation analysis method based upon a wild-type DNA sequence is presented. Oligonucleotides were utilized for primer extension by T7 DNA polymerase to discriminate between wild-type and mutant sequences in two solid phase approaches. 1. Oligonucleotides were annealed to an immobilized template, extended with fluorescent dideoxynucleotides (ddNTPs), and analyzed on an automated fluorescent DNA sequencer. The oligonucleotide length identified the known mutation site, and the fluorescence emission of the ddNTP identified the mutation. 2. Template DNA was annealed to an oligonucleotide array, extended with α-32P dNTPs, and analyzed with a Phosphor Imager. The grid position of the oligonucleotide identified the mutation site and the extended base identified the mutation. © 1996 Wiley-Liss, Inc.
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