Congo red- and Zn(II)-derivatized monosize poly(MMA-HEMA) microspheres as specific sorbent in metal chelate affinity of albumin

Monosize poly(methylmethacrylate-hydroxyethylmethacrylate) [poly(MMA-HEMA)] microspheres (4 μm in diameter) were produced by dispersion copolymerization of MMA and HEMA in an ethanol-water medium. Congo Red was attached to the poly(MMA-HEMA) microspheres, covalently. These Congo Red-derivatized microspheres were characterized by optical microscopy, Fourier transform infrared spectroscopy, and elemental analysis. Then, Zn(II) ions were incorporated by chelating with the immobilized Congo Red molecules. Different amounts of Zn(II) ions [1.2–17.6 mg of Zn(II)/g of polymer] were conjugated on the microspheres by changing the initial concentration of Zn(II) ions and pH. Bovine serum albumin (BSA) adsorption on these microspheres from aqueous solutions containing different amounts of BSA at different pH and ionic strengths was investigated in batch reactors. The nonspecific BSA adsorption on the plain poly(MMA-HEMA) microspheres was very low (0.7 mg of BSA/g of polymer). Congo Red derivatization significantly increased the BSA adsorption (up to 35.8 mg of BSA/g of polymer). A further increase in the adsorption capacity (up to 61.0 mg of BSA/g of polymer) was observed when Zn(II) ions were incorporated. More than 90% of the adsorbed BSA was desorbed in 1 h in the desorption medium containing 1.0M NaSCN at pH 8.0. © 1997 John Wiley & Sons, Inc.