Effects of inactivity on myosin heavy chain composition and size of rat soleus fibers
Elena J. Grossman BS
Department of Physiological Science, University of California, Los Angeles, Los Angeles, California 90095, USA
Search for more papers by this authorCorresponding Author
Roland R. Roy PhD
Brain Research Institute, UCLA School of Medicine, Center for the Health Sciences, 10833 Le Conte Avenue, Los Angeles, California 90095-1761, USA
Brain Research Institute, UCLA School of Medicine, Center for the Health Sciences, 10833 Le Conte Avenue, Los Angeles, California 90095-1761, USASearch for more papers by this authorRobert J. Talmadge PhD
Department of Physiological Science, University of California, Los Angeles, Los Angeles, California 90095, USA
Search for more papers by this authorHui Zhong MD
Brain Research Institute, UCLA School of Medicine, Center for the Health Sciences, 10833 Le Conte Avenue, Los Angeles, California 90095-1761, USA
Search for more papers by this authorV. Reggie Edgerton PhD
Department of Physiological Science, University of California, Los Angeles, Los Angeles, California 90095, USA
Brain Research Institute, UCLA School of Medicine, Center for the Health Sciences, 10833 Le Conte Avenue, Los Angeles, California 90095-1761, USA
Search for more papers by this authorElena J. Grossman BS
Department of Physiological Science, University of California, Los Angeles, Los Angeles, California 90095, USA
Search for more papers by this authorCorresponding Author
Roland R. Roy PhD
Brain Research Institute, UCLA School of Medicine, Center for the Health Sciences, 10833 Le Conte Avenue, Los Angeles, California 90095-1761, USA
Brain Research Institute, UCLA School of Medicine, Center for the Health Sciences, 10833 Le Conte Avenue, Los Angeles, California 90095-1761, USASearch for more papers by this authorRobert J. Talmadge PhD
Department of Physiological Science, University of California, Los Angeles, Los Angeles, California 90095, USA
Search for more papers by this authorHui Zhong MD
Brain Research Institute, UCLA School of Medicine, Center for the Health Sciences, 10833 Le Conte Avenue, Los Angeles, California 90095-1761, USA
Search for more papers by this authorV. Reggie Edgerton PhD
Department of Physiological Science, University of California, Los Angeles, Los Angeles, California 90095, USA
Brain Research Institute, UCLA School of Medicine, Center for the Health Sciences, 10833 Le Conte Avenue, Los Angeles, California 90095-1761, USA
Search for more papers by this authorAbstract
Myosin heavy chain (MHC) and fiber size properties of the adult rat soleus were determined after 4–60 days of complete inactivity, i.e., lumbar spinal cord isolation. Soleus atrophy was rapid and progressive, i.e., 25% and 64% decrease in weight and 33% and 75% decrease in fiber size after 4 and 60 days of inactivity, respectively. Changes in MHC occurred at a slower rate than the atrophic response. After 15 days there was de novo expression of type IIx MHC (∼10%). By 60 days, type IIx MHC accounted for 33% of the total MHC content, and 7% of the fibers contained only type IIx MHC. The relative amount of type I MHC was reduced from 93% in control to 49% after 60 days of inactivity. Therefore, the effects of 60 days of inactivity suggest that during this time period at least 75% of fiber size and ∼40% of type I MHC composition of the adult rat soleus can be attributed to activation-related events. © 1998 John Wiley & Sons, Inc. Muscle Nerve 21:375–389, 1998.
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