Lack of type 1 and type 2A myosin heavy chain isoforms in rat slow muscle regenerating during chronic nerve block
Corresponding Author
Menotti Midrio MD
Istituto di Fisiologia Umana, Università di Padova, Via Marzolo 3, 35131, Padua, Italy
Istituto di Fisiologia Umana, Università di Padova, Via Marzolo 3, 35131, Padua, ItalySearch for more papers by this authorDaniela Danieli-Betto DBiol
Istituto di Fisiologia Umana, Università di Padova, Via Marzolo 3, 35131, Padua, Italy
Search for more papers by this authorAlessandra Esposito DBiol
Istituto di Fisiologia Umana, Università di Padova, Via Marzolo 3, 35131, Padua, Italy
Search for more papers by this authorAram Megighian MD
Istituto di Fisiologia Umana, Università di Padova, Via Marzolo 3, 35131, Padua, Italy
Search for more papers by this authorUgo Carraro MD
Dipartimento di Scienze Biomediche Sperimentali, Università di Padova, Via G. Colombo, 3, 35121, Padua, Italy
Search for more papers by this authorClaudia Catani DBiol
Dipartimento di Scienze Biomediche Sperimentali, Università di Padova, Via G. Colombo, 3, 35121, Padua, Italy
Search for more papers by this authorKatia Rossini DBiol
Dipartimento di Scienze Biomediche Sperimentali, Università di Padova, Via G. Colombo, 3, 35121, Padua, Italy
Search for more papers by this authorCorresponding Author
Menotti Midrio MD
Istituto di Fisiologia Umana, Università di Padova, Via Marzolo 3, 35131, Padua, Italy
Istituto di Fisiologia Umana, Università di Padova, Via Marzolo 3, 35131, Padua, ItalySearch for more papers by this authorDaniela Danieli-Betto DBiol
Istituto di Fisiologia Umana, Università di Padova, Via Marzolo 3, 35131, Padua, Italy
Search for more papers by this authorAlessandra Esposito DBiol
Istituto di Fisiologia Umana, Università di Padova, Via Marzolo 3, 35131, Padua, Italy
Search for more papers by this authorAram Megighian MD
Istituto di Fisiologia Umana, Università di Padova, Via Marzolo 3, 35131, Padua, Italy
Search for more papers by this authorUgo Carraro MD
Dipartimento di Scienze Biomediche Sperimentali, Università di Padova, Via G. Colombo, 3, 35121, Padua, Italy
Search for more papers by this authorClaudia Catani DBiol
Dipartimento di Scienze Biomediche Sperimentali, Università di Padova, Via G. Colombo, 3, 35121, Padua, Italy
Search for more papers by this authorKatia Rossini DBiol
Dipartimento di Scienze Biomediche Sperimentali, Università di Padova, Via G. Colombo, 3, 35121, Padua, Italy
Search for more papers by this authorAbstract
The degeneration regeneration process was induced by bupivacaine injection in innervated, denervated, and nerve-blocked rat soleus muscles. Nerve block was obtained by superfusion of the sciatic nerve with tetrodotoxin (TTX). Two weeks after bupivacaine injection, immunohistochemical and electrophoretical analyses showed the presence of type 1 myosin heavy chain (MHC) only in innervated regenerated muscles, type 2A in innervated and denervated, but not in TTX-paralyzed muscles, and type 2X under all experimental conditions. The presence of type 1 MHC in the innervated, and its absence in both denervated and TTX-paralyzed muscles were also verified immunohistochemically 1 week after bupivacaine injection. It is concluded that the nerve impulses play a determinant role in the expression of 1 and 2A MHC isoforms in the innervated regenerating muscle. The possible causes of the absence of the type 2A MHC isoform in the TTX-paralyzed muscles are discussed. © 1998 John Wiley & Sons, Inc. Muscle Nerve 21: 226–232, 1998
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