Experimental Cancer

Peritoneal fluid from ovarian cancer patients stimulates MUC1 epithelial mucin expression in ovarian cancer cell lines

David J. Munster

Department of Obstetrics and Gynaecology, The University of Queensland, Clinical Sciences Building, Royal Brisbane Hospital, Herston, Australia

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Rachel J. Quin,

Rachel J. Quin

Department of Obstetrics and Gynaecology, The University of Queensland, Clinical Sciences Building, Royal Brisbane Hospital, Herston, Australia

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Amolak S. Bansal,

Amolak S. Bansal

Department of Medicine, The University of Queensland, Princess Alexandra Hospital, Wooloongabba, Australia

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Bruce G. Ward,

Bruce G. Ward

Department of Obstetrics and Gynaecology, The University of Queensland, Clinical Sciences Building, Royal Brisbane Hospital, Herston, Australia

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Michael A. McGuckin,

Corresponding Author

Michael A. McGuckin

[email protected]

Department of Obstetrics and Gynaecology, The University of Queensland, Clinical Sciences Building, Royal Brisbane Hospital, Herston, Australia

Department of Obstetrics and Gynaecology, The University of Queensland, Clinical Sciences Building, Royal Brisbane Hospital, Herston 4029, Australia. Fax: (61)7-33655199Search for more papers by this author

David J. Munster,

David J. Munster

Department of Obstetrics and Gynaecology, The University of Queensland, Clinical Sciences Building, Royal Brisbane Hospital, Herston, Australia

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Rachel J. Quin,

Rachel J. Quin

Department of Obstetrics and Gynaecology, The University of Queensland, Clinical Sciences Building, Royal Brisbane Hospital, Herston, Australia

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Amolak S. Bansal,

Amolak S. Bansal

Department of Medicine, The University of Queensland, Princess Alexandra Hospital, Wooloongabba, Australia

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Bruce G. Ward,

Bruce G. Ward

Department of Obstetrics and Gynaecology, The University of Queensland, Clinical Sciences Building, Royal Brisbane Hospital, Herston, Australia

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Michael A. McGuckin,

Corresponding Author

Michael A. McGuckin

[email protected]

Department of Obstetrics and Gynaecology, The University of Queensland, Clinical Sciences Building, Royal Brisbane Hospital, Herston, Australia

First published: 06 December 1998

https://doi.org/10.1002/(SICI)1097-0215(19980504)76:3<393::AID-IJC17>3.0.CO;2-C

Citations: 3

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Abstract

The MUC1 epithelial mucin is a transmembrane glycoprotein that is frequently but variably over-expressed by adenocarcinomas. It is used as a diagnostic serum tumour marker and is a candidate target for tumour immunotherapy. Peritoneal fluid (PF) samples from ovarian cancer patients were investigated for their ability to modulate MUC1 expression in 6 ovarian cancer cell lines which showed a range from very low to high endogenous MUC1 expression. Cell lines were cultured in 20% PF for 4 days, fixed in situ and MUC1 assayed by ELISA. MUC1 expression was stimulated by some PF samples in 5 of 6 lines tested. MUC1 expression in the PE04 cell line (very low endogenous expression) was increased by 35 of 36 PFs tested (p < 0.05); stimulation varied between PFs but was greater than with 100 IU/mL hu-r-γ-interferon. Western blotting confirmed the stimulation of MUC1 in PE04 cells and FACS showed an increase in the proportion of cells expressing MUC1. The active factor was partially purified by gel filtration and was shown to stimulate PE04 cells in a dose-dependent manner. Concentrations of IL1β, IL4, IL6, IL8, IL10, TNF-α, TGF-β and GM-CSF were often very high in PF and varied substantially between different PF samples but did not correlate with the degree of MUC1 stimulatory activity. Int. J. Cancer 76:393–398, 1998.© 1998 Wiley-Liss, Inc.

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Volume76, Issue3

4 May 1998

Pages 393-398

Peritoneal fluid from ovarian cancer patients stimulates MUC1 epithelial mucin expression in ovarian cancer cell lines

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Peritoneal fluid from ovarian cancer patients stimulates MUC1 epithelial mucin expression in ovarian cancer cell lines

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