Experimental Cancer

MIBG causes oxidative stress and up-regulation of anti-oxidant enzymes in the human neuroblastoma cell line SK-N-BE(2C)

Jan Cornelissen

Academic Medical Centre, Departments of Paediatrics and Clinical Chemistry, PO Box 22700, 1100 DE Amsterdam, The Netherlands

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André B.P. Van Kuilenburg,

André B.P. Van Kuilenburg

Academic Medical Centre, Departments of Paediatrics and Clinical Chemistry, PO Box 22700, 1100 DE Amsterdam, The Netherlands

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P.A. Voûte,

P.A. Voûte

Academic Medical Centre, Departments of Paediatrics and Clinical Chemistry, PO Box 22700, 1100 DE Amsterdam, The Netherlands

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Albert H. Van Gennip,

Corresponding Author

Albert H. Van Gennip

[email protected]

Academic Medical Centre, Departments of Paediatrics and Clinical Chemistry, PO Box 22700, 1100 DE Amsterdam, The Netherlands

Fax: +31-20-6962596Search for more papers by this author

Jan Cornelissen,

Jan Cornelissen

Academic Medical Centre, Departments of Paediatrics and Clinical Chemistry, PO Box 22700, 1100 DE Amsterdam, The Netherlands

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André B.P. Van Kuilenburg,

André B.P. Van Kuilenburg

Academic Medical Centre, Departments of Paediatrics and Clinical Chemistry, PO Box 22700, 1100 DE Amsterdam, The Netherlands

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P.A. Voûte,

P.A. Voûte

Academic Medical Centre, Departments of Paediatrics and Clinical Chemistry, PO Box 22700, 1100 DE Amsterdam, The Netherlands

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Albert H. Van Gennip,

Corresponding Author

Albert H. Van Gennip

[email protected]

Academic Medical Centre, Departments of Paediatrics and Clinical Chemistry, PO Box 22700, 1100 DE Amsterdam, The Netherlands

Fax: +31-20-6962596Search for more papers by this author

First published: 06 December 1998

https://doi.org/10.1002/(SICI)1097-0215(19970729)72:3<486::AID-IJC17>3.0.CO;2-A

Citations: 9

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Abstract

We report the effects of meta-iodobenzylguanidine (MIBG), a neuroblastoma-seeking agent, on cell proliferation and several oxidative stress-related parameters in the human neuroblastoma cell line SK-N-BE(2c). MIBG inhibited the proliferation of this cell line in micromolar concentrations. Measurements of the malondialdehyde (MDA) concentrations (a measure of the extent of lipid peroxidation) of cells treated with MIBG showed that increasing concentrations of MIBG led to an increase in MDA levels of the cells. This effect was most pronounced after one day of cellular exposure to MIBG and disappeared after 3 days. Disappearance of the elevated MDA levels caused by MIBG is probably the result of increased activity of the H₂O₂ detoxifying enzymes, catalase and glutathion peroxidase (GPx). The catalase- and GPx-enzyme activity of cells exposed to MIBG steadily increased with time, reaching a maximum after 4 days. Oxidative stress caused by MIBG thus at first leads to cellular damage (lipid peroxidation) but over a longer period does not lead to decreased proliferation rate of the cells, most likely because of cellular adaptation to increased oxidative stress by up-regulation of the H₂O₂ detoxifying enzymes catalase and GPx. Int. J. Cancer 72:486–490, 1997. © 1997 Wiley-Liss, Inc.

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Volume72, Issue3

29 July 1997

Pages 486-490

MIBG causes oxidative stress and up-regulation of anti-oxidant enzymes in the human neuroblastoma cell line SK-N-BE(2C)

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MIBG causes oxidative stress and up-regulation of anti-oxidant enzymes in the human neuroblastoma cell line SK-N-BE(2C)

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