Molecular cloning and sequencing of a chitin synthase gene (CHS2) of Paracoccidioides brasiliensis
Gustavo A. Niño-Vega
Department of Molecular & Cell Biology, Institute of Medical Sciences, University of Aberdeen, Foresterhill, Aberdeen AB25 2ZD, U.K.
Search for more papers by this authorEd T. Buurman
Department of Molecular & Cell Biology, Institute of Medical Sciences, University of Aberdeen, Foresterhill, Aberdeen AB25 2ZD, U.K.
Search for more papers by this authorGraham W. Gooday
Department of Molecular & Cell Biology, Institute of Medical Sciences, University of Aberdeen, Foresterhill, Aberdeen AB25 2ZD, U.K.
Search for more papers by this authorGioconda San-Blas
Instituto Venezolano de Investigaciones Científicas, Centro de Microbiología y Biología Celular, Apartado 21827, Caracas 1020-A, Venezuela
Search for more papers by this authorCorresponding Author
Neil A. R. Gow
Department of Molecular & Cell Biology, Institute of Medical Sciences, University of Aberdeen, Foresterhill, Aberdeen AB25 2ZD, U.K.
Department of Molecular & Cell Biology, Institute of Medical Sciences, University of Aberdeen, Foresterhill, Aberdeen AB25 2ZD, U.K.Search for more papers by this authorGustavo A. Niño-Vega
Department of Molecular & Cell Biology, Institute of Medical Sciences, University of Aberdeen, Foresterhill, Aberdeen AB25 2ZD, U.K.
Search for more papers by this authorEd T. Buurman
Department of Molecular & Cell Biology, Institute of Medical Sciences, University of Aberdeen, Foresterhill, Aberdeen AB25 2ZD, U.K.
Search for more papers by this authorGraham W. Gooday
Department of Molecular & Cell Biology, Institute of Medical Sciences, University of Aberdeen, Foresterhill, Aberdeen AB25 2ZD, U.K.
Search for more papers by this authorGioconda San-Blas
Instituto Venezolano de Investigaciones Científicas, Centro de Microbiología y Biología Celular, Apartado 21827, Caracas 1020-A, Venezuela
Search for more papers by this authorCorresponding Author
Neil A. R. Gow
Department of Molecular & Cell Biology, Institute of Medical Sciences, University of Aberdeen, Foresterhill, Aberdeen AB25 2ZD, U.K.
Department of Molecular & Cell Biology, Institute of Medical Sciences, University of Aberdeen, Foresterhill, Aberdeen AB25 2ZD, U.K.Search for more papers by this authorAbstract
The nucleotide sequence of a chitin synthase gene (CHS2) of the dimorphic fungal human pathogen Paracoccidioides brasiliensis has been determined. The deduced amino acid sequence of Chs2p consists of 1043 residues and is highly homologous to other class II fungal chitin synthases. Computational structural analyses suggest very high similarity to other fungal chitin synthases with a highly variable region at the cytosolic amino-terminal region which may be related to its possible zymogenic nature, and the putative catalytic region close to seven membrane-spanning regions at the carboxyl terminus. The nucleotide sequence of CHS2 and its flanking regions has been submitted to GenBank under Accession Number Y09231. © 1998 John Wiley & Sons, Ltd.
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