High prevalence of hepatitis G viremia among kidney transplant patients in Thailand
Corresponding Author
Boonyos Raengsakulrach
Department of Virology, U.S. Army Medical Component, Armed Forces Research Institute of Medical Sciences, Bangkok, Thailand
Department of Virology, USAMC-AFRIMS, APO AP 96546===Search for more papers by this authorLeena Ong-aj-yooth
Renal Unit, Department of Medicine, Faculty of Medicine, Siriraj Hospital, Mahidol University, Bangkok, Thailand
Search for more papers by this authorThanarak Thaiprasert
Department of Biochemistry, Faculty of Medicine, Siriraj Hospital, Mahidol University, Bangkok, Thailand
Search for more papers by this authorSanga Nilwarangkur
Renal Unit, Department of Medicine, Faculty of Medicine, Siriraj Hospital, Mahidol University, Bangkok, Thailand
Search for more papers by this authorSompong Ong-aj-yooth
Department of Biochemistry, Faculty of Medicine, Siriraj Hospital, Mahidol University, Bangkok, Thailand
Search for more papers by this authorSumitda Narupiti
Department of Virology, U.S. Army Medical Component, Armed Forces Research Institute of Medical Sciences, Bangkok, Thailand
Search for more papers by this authorVipa Thirawuth
Department of Virology, U.S. Army Medical Component, Armed Forces Research Institute of Medical Sciences, Bangkok, Thailand
Search for more papers by this authorChonticha Klungthong
Department of Virology, U.S. Army Medical Component, Armed Forces Research Institute of Medical Sciences, Bangkok, Thailand
Search for more papers by this authorRapin Snitbhan
Department of Virology, U.S. Army Medical Component, Armed Forces Research Institute of Medical Sciences, Bangkok, Thailand
Search for more papers by this authorDavid W. Vaughn
Department of Virology, U.S. Army Medical Component, Armed Forces Research Institute of Medical Sciences, Bangkok, Thailand
Search for more papers by this authorCorresponding Author
Boonyos Raengsakulrach
Department of Virology, U.S. Army Medical Component, Armed Forces Research Institute of Medical Sciences, Bangkok, Thailand
Department of Virology, USAMC-AFRIMS, APO AP 96546===Search for more papers by this authorLeena Ong-aj-yooth
Renal Unit, Department of Medicine, Faculty of Medicine, Siriraj Hospital, Mahidol University, Bangkok, Thailand
Search for more papers by this authorThanarak Thaiprasert
Department of Biochemistry, Faculty of Medicine, Siriraj Hospital, Mahidol University, Bangkok, Thailand
Search for more papers by this authorSanga Nilwarangkur
Renal Unit, Department of Medicine, Faculty of Medicine, Siriraj Hospital, Mahidol University, Bangkok, Thailand
Search for more papers by this authorSompong Ong-aj-yooth
Department of Biochemistry, Faculty of Medicine, Siriraj Hospital, Mahidol University, Bangkok, Thailand
Search for more papers by this authorSumitda Narupiti
Department of Virology, U.S. Army Medical Component, Armed Forces Research Institute of Medical Sciences, Bangkok, Thailand
Search for more papers by this authorVipa Thirawuth
Department of Virology, U.S. Army Medical Component, Armed Forces Research Institute of Medical Sciences, Bangkok, Thailand
Search for more papers by this authorChonticha Klungthong
Department of Virology, U.S. Army Medical Component, Armed Forces Research Institute of Medical Sciences, Bangkok, Thailand
Search for more papers by this authorRapin Snitbhan
Department of Virology, U.S. Army Medical Component, Armed Forces Research Institute of Medical Sciences, Bangkok, Thailand
Search for more papers by this authorDavid W. Vaughn
Department of Virology, U.S. Army Medical Component, Armed Forces Research Institute of Medical Sciences, Bangkok, Thailand
Search for more papers by this authorAbstract
Patients receiving kidney transplants (KT) are at high risk for blood borne viral infections. To determine the prevalence of a recently discovered hepatitis G virus (HGV) in this patient group, reverse transcription-polymerase chain reaction (RT-PCR) employing primers derived from the NS5 region of the viral genome was utilized. HGV RNA was detected in 40 of 94 KT patients (43%), as compared to 3 of 69 healthy subjects (4.3%). Cocirculation of HGV and hepatitis C virus (HCV) RNA was detected in 12 patients (13%). Comparison of patients with and without HGV revealed that the former had received hemodialysis before transplantation for a significantly longer duration than the latter (28 vs. 17 months, respectively; P < 0.05). The amount of blood transfused and mean levels of liver enzymes, including alkaline phosphatase, alanine transaminase, and aspartate transaminase, were the same in both groups. Sequence analysis of 275-base pair DNA clones obtained from 2 patients revealed approximately 92% sequence homology to the published HGV and GB virus C sequences. These results suggested that HGV infection among Thai KT patients was high and the role of HGV in causing liver disease remains to be determined. J. Med. Virol. 53:162–166, 1997. © 1997 Wiley-Liss, Inc.
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